电针胃俞募穴对大鼠胃运动及延髓DVC区c-fos表达的影响  

Effect of Electroacupuncture at Point Weishu and Front-Mu points on Rat Gastric Motility and c-fos Expression in Bulbar DVC Area

作　　者：申国明[1] 王浩[1] 秦勇[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,合肥230038

出　　处：《上海针灸杂志》2012年第5期357-359,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81072871);安徽省自然科学基金(090413115);国家重点基础研究发展计划(2011CB505205)

摘　　要：目的观察电针胃俞募穴对大鼠胃运动的影响,并探讨其中枢作用机制。方法选用成年SD大鼠32只,随机分为空白组、中脘组、胃俞组、中脘+胃俞组,每组8只。采用自制应力传感器记录各组大鼠胃窦部胃运动波形,并应用免疫组织化学染色的方法,检测迷走背核复合体(dorsal vagal complex,DVC)中原癌基因c-fos的表达。结果与空白组比较,其余各组DVC中c-fos表达及胃运动幅度均明显增加(均P<0.01),且中脘+胃俞组c-fos表达及胃运动幅度的提高与胃俞组和中脘组比较,差异均具有统计学意义(均P<0.05)。结论电针胃俞募穴对胃运动具有调节作用,这种调节作用可能是通过激活延髓DVC区神经元实现的,且俞募配穴具有协同效应。Objective To investigate the effect of electroacupuncture at point Weishu and Front,Mu points on rat gastric motility and explore the mechanism of its central action. Method Thirty-two adult SD rats were randomlY allocated to blank, Zhongwan, Weishu and Zhongwan plus Weishu groups, 8 rats each. Gastric motility waveforms were recorded using a self-made strain transducer in different groups of rats. The expression of proto oncogene c-los in the dorsal vagat complex was examined by. Result Compared with the blank group, DVC expression of c-los and gastric motility amplitude increased significantly in the other groups （all P〈0.01）. There were statistically significant differences in increases in c-los expression and gastric motility amplitude between Zhongwan plus Weishu group and Weishu group or Zhongwan group （both P〈0.05）. conclusion Electroacupuncture at point Weishu and Front-Mu points has a regulating effect on gastric motility. This regulating effect is achieved by activating neurons in bulbar DVC area. Baek-Shu-Front-Mu point combination has a synergistic effect.

关 键 词：电针 胃运动 迷走背核复合体 大鼠 

分 类 号：R2-03[医药卫生—中医学]