壳寡糖作用于巨噬细胞的机制初探  

Mechanisms of Chitosan Oligosaccharides Acting on Macrophage

作　　者：麻攀[1,2] 于炜婷[1,2] 魏鹏[1,2] 许青松[1] 白雪芳[1] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所1805组,大连116023 [2]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2012年第5期660-662,共3页Natural Product Research and Development

基　　金：国家科技重大专项课题(2009ZX095010)新药研究开发关键技术研究

摘　　要：研究壳寡糖对免疫系统中巨噬细胞作用的具体机制。结合流式细胞仪和激光共聚焦实验检测壳寡糖与巨噬细胞相互作用,通过凝胶阻滞实验在体外验证壳寡糖的胞内定位。实验结果证明壳寡糖与巨噬细胞相互作用过程如下,先与细胞膜结合,然后进入细胞内,最后定位在细胞核的核酸(DNA/RNA)上。The molecular mechanisms of chitosan oligosacchrides （COS） acting on macrophages in immune system were investigated here. Through Laser Scanning Confocal Microscopy （LSCM） and Flow cytometric analysis, we examined the interaction between COS and macrophages. Gel-retardation experiment was used to investigate the location of COS in the nucleus. The results suggested that the interaction between COS and macrophages was followed in proper sequence. First, COS bound to the plasma membrane of macrophage cells before entering into the cytoplasm, and lastly, it was loca- ted on DNA/RNA in the nucleus.

关 键 词：壳寡糖 巨噬细胞 DNA RNA 

分 类 号：Q26[生物学—细胞生物学]