RP-HPLC法测定抗癌新药WF210的含量和有关物质  

Determination of New Anticancer Drug WF210 and Its Related Substances by RP-HPLC

作　　者：王曼曼[1] 霍艳双[1] 李清[1] 崔红霞[1] 陈晓辉[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学,沈阳110016

出　　处：《中国药房》2012年第21期1990-1992,共3页China Pharmacy

摘　　要：目的:建立测定抗癌新药乙酰肼类化合物WF210的含量及有关物质的方法。方法:采用反相高效液相色谱法。色谱柱为Diamonsil C18,流动相为乙腈-甲醇-水(60∶20∶20),流速为1·0mL·min-1,紫外检测波长为238nm,柱温为30℃。结果:WF210检测浓度线性范围为5·045～50·45μg·mL-1(r=0·9998),平均回收率为100·0%(RSD=0·5%);主成分与其有关物质分离良好,检测限为0·8ng。结论:该方法简便、准确、重复性好,可用于WF210的含量测定和有关物质的检查。OBJECTIVE：To establish a method for the content determination of new anticancer drug WF210 and its related substances.METHODS：RP-HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-methanol-water（60：20：20）at the flow rate of 1.0 mL·min-1.UV detection wavelength was set at 238 nm and column temperature was 30 ℃.RESULTS：The linear range of WF210 was 5.045～50.45 μg·mL-1（r=0.999 8） with an average recovery of 100.0%（RSD=0.5%）.The related substances of WF210 could be completely separated from WF210.The limit of detection was 0.8 ng.CONCLUSION：The method is simple,accurate and reproducible for the content determination of WF210 and its related substances.

关 键 词：WF210 高效液相色谱法 含量测定 有关物质 

分 类 号：R927.2[医药卫生—药学] R979.1