牡丹‘乌龙捧盛’不定芽的诱导及增殖  

Multiplication and Induction of Adventitious Buds of Tree Peony 'Wulongpengsheng'

作　　者：徐小博[1] 贾文庆[1] 刘会超[1] 郑健[1] 

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003

出　　处：《北方园艺》2012年第11期127-130,共4页Northern Horticulture

基　　金：河南省创新人才工程资助项目(2005-126-49)

摘　　要：以牡丹品种‘乌龙捧盛’侧芽为试材,研究了MS和WPM 2种基本培养基、不同植物生长调节剂配比、不同蔗糖浓度和继代次数对不定芽诱导和增殖的影响。结果表明:WPM培养基对侧芽萌发和不定芽的诱导效果优于MS;不定芽增殖的最佳生长调节剂的组合是6-BA2.0mg/L+NAA 0.3mg/L,增殖率最高达811.23%;蔗糖浓度为35mg/L时玻璃化率最低,更有利于不定芽的增殖;随着继代次数增加,不定芽增殖率先升后降,继代次数为4次时不定芽增殖率达最高。Tree peony’Wulongpengsheng’s lateral buds were used as explants,the effect of basic medium,plant growth regulation,the concentration of sucrose,germination times on the adventitious buds differentiation were studied..The results showed that WPM was better than MS.The best medium for proliferation was WPM＋6-BA 2.0 mg/L＋IAA 0.3 mg/L,with the proliferation of was 811.13%.The best concentration of sucrose was 35 mg/L.With the germination times increasing,the proliferation rose first and fell later.The proliferation rate reached highest at the fourth.

关 键 词：牡丹 不定芽 继代次数 

分 类 号：S685.11[农业科学—观赏园艺]