壳寡糖对S180荷瘤鼠Bcl-2、Bax蛋白表达的影响  

The effects of chitosan oligosaccharide on the protein expression of Bcl-2,Bax of S180 tumor cell in mice

作　　者：蒋瑞雪[1] 赵宪 孙艳[1] 王琪[1] 兴桂华[1] 吴艳敏[1] 官杰[1] 

机构地区：[1]齐齐哈尔医学院医学技术学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔第一医院介入科,黑龙江齐齐哈尔161000

出　　处：《中国医药导报》2012年第13期27-28,共2页China Medical Herald

基　　金：黑龙江省教育厅科学技术研究项目(项目编号:12511626);齐齐哈尔市科学技术计划项目

摘　　要：目的观察壳寡糖对S180小鼠Bcl-2、Bax蛋白表达情况及肿瘤细胞凋亡的影响。方法选择体内注射的方法将壳寡糖注入S180小鼠体内,用免疫组织化学法测定瘤组织Bcl-2、Bax蛋白的表达情况。结果壳寡糖能降低S180小鼠瘤组织抑凋亡基因Bcl-2的表达,提高促凋亡基因Bax的表达。结论通过调节Bcl-2、Bax的表达而诱使肿瘤细胞凋亡是壳寡糖抗肿瘤作用机制之一。Objective To observe the effects of chitosan oligosaccharide on the protein expression of Bcl-2,Bax and the apoptosis of tumor cell of S180 tumor cell in mice.Methods The expression of apoptosis-related proteins（Bcl-2 and Bax） was determined by the method of immunohistochemistry after chitosan oligosaccharide was injected into the mice-bearing tumor.Results The expression of antiapoptotic protein Bcl-2 was reduced and the expression of proapoptotic protein Bax was increased by chitosan oligosaccharide.Conclusion Inducing the tumor cell apoptosis is one of the mechanism of action on chitosan oligosaccharide anti-tumor by regulating the expression of Bcl-2,Bax protein.

关 键 词：壳寡糖 S180小鼠 BCL-2 BAX 凋亡 

分 类 号：R392[医药卫生—免疫学]