在线柱浓缩-液相色谱法测定水体中痕量敌草快和百草枯  

Determination of trace diquat and paraquat in water by online column enrichment-high performance liquid chromatography

作　　者：陈艳[1] 罗继 袁斌 刘绿叶 张艳海 

机构地区：[1]浙江省环境保护科学设计研究院,杭州310028 [2]赛默飞世尔科技,上海201203

出　　处：《环境化学》2012年第5期748-751,共4页Environmental Chemistry

摘　　要：采用在线柱浓缩-超快速液相色谱联用技术测定水体中痕量百草枯和敌草快.水样无需样品前处理,过滤后即可进样.采用固相萃取小柱富集待测物,以色谱梯度泵完成样品的净化后,利用阀切换技术将待测物反冲至分析柱进行分离,以二极管阵列检测器定量.方法在1.0—20.0μg.L-1范围内线性良好,百草枯和敌草快的线性相关系数分别为0.9997和0.9989.百草枯和敌草快的检出限(S/N=3)分别为0.10、0.12μg.L-1,加标回收率在96.0%—98.0%之间.用所建立的方法测定了水中痕量的百草枯与敌草快的含量,结果令人满意.A novel online column enrichment-high performance liquid chromatography（HPLC）method was developed to determine trace diquat and paraquat in water.The sample was injected into HPLC directly after filtrated with 0.45 μm membrane,and then the analytes were collected by the online solid phase extraction（SPE） column using loading pump.The collection was back-flushed into the analytical column using another pump by valve switching method.Diquat and paraquat were performed by diode array detector（DAD）.The linear ranges of diquat and paraquat were 1.0—20.0 μg·L-1,the correlation coefficients of the two analytes were 0.9997 and 0.9989,and the limits of detection（S/N=3） were 0.10 μg·L-1,0.12 μg·L-1,respectively.The average recoveries were 96.0%—98.0%.The method was applied to determine trace diquat and paraquat in spiked water samples with satisfactory results.

关 键 词：在线浓缩 百草枯 敌草快 液相色谱 双梯度 

分 类 号：X832[环境科学与工程—环境工程] O657.72[理学—分析化学]