海参皂苷Echinoside A和Ds-echinoside A对C57BL/6小鼠黑色素瘤B16自发性肺转移的影响  

Inhibitory Effect of Echinoside A and Ds-Echinoside A on Spontaneous Lung Metastases of B16 Melanoma Cells in C57BL/6 Mice

作　　者：赵芹[1] 薛长湖[1] 杨玉红[1] 薛勇[1] 王玉明[1] 李兆杰[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2012年第9期230-234,共5页Food Science

基　　金：海洋公益性行业科研专项(201105029);国际科技合作项目(2010DFA31330);国家自然科学基金项目(30972284);"泰山学者"建设工程专项

摘　　要：目的:通过建立小鼠黑色素瘤B16细胞实验性肺转移模型,探讨海参皂苷Echinoside A(EA)和Ds-echinoside A(DSEA)的体内抑制肿瘤肺转移作用。方法:连续腹腔注射EA和DSEA 25d后,检测小鼠肺转移灶形成,血清中唾液酸(SA)含量、γ-谷氨酰转肽酶(γ-GT)活力和肺组织中羟脯氨酸、氨基己糖、糖醛酸的含量。结果:EA和DSEA能显著减少小鼠B16细胞肺转移灶的数目,其中高剂量组的转移抑制率分别为87.07%和74.14%(P<0.01)。EA和DSEA均能降低血清中SA含量和γ-GT活力以及肺组织中羟脯氨酸、氨基己糖和糖醛酸含量(P<0.05,P<0.01)。结论:EA和DSEA均能显著抑制黑色素瘤细胞的增殖活性和运动性,从而显著抑制肿瘤细胞在小鼠体内的转移和生长。Objective： An artificial lung metastatic mouse model was established by intravenous injection of B16 melanoma cells and the anti-metastatic effect of echinoside A （EA） and ds-echinoside A （DSEA） was investigated. Methods： B 16 artificial lung metastatic mice were treated every other day by intraperitoneally injection of EA or DSEA for 25 consecutive days. The mice were then sacrificed and the blood was collected for separating serum. The lungs were dissected to detect metastatic colonies. The serum levels of sialic acids, y-glutamyltranspeptidase （g-GT） and hepatic hydroxyproline, hexosamines, and uronic acid contents were measured. Results： EA and DSEA could reduce the quantity of B 16 metastasis. High-dose EA and DSEA could inhibit the metastasis by 87.07% and 74.14% （P〈0.01）, respectively. The content of serum saliva acid and y-GT activity were also reduced by both EA and DSEA significantly （P 〈 0.05, P 〈 0.01）. Biochemical parameters such as hydroxyproline, hexosamines and uronic acid levels in the lung were also significantly reduced by EA and DSEA （P〈0.05, P〈0.01）. Conclusion： EA and DSEA can inhibit artificial lung metastases of B 16 melanoma cells by inhibiting tumor cell migration and reduce the degree of lung fibrosis.

关 键 词：echinoside A ds-echinoside A 自发性肺转移 Γ-谷氨酰转肽酶 羟脯氨酸 氨基己糖 

分 类 号：R965[医药卫生—药理学]