纳米TiO_2光催化氧化对胃癌细胞周期的影响  

Effects of Nano-TiO_2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells

作　　者：文雯[1] 高晓亚[1] 张爱平[1] 宋志英[1] 孙彦平[2] 

机构地区：[1]山西医科大学药学院,太原030001 [2]太原理工大学洁净化工研究所,太原030024

出　　处：《应用化学》2012年第6期663-667,共5页Chinese Journal of Applied Chemistry

基　　金：山西省自然科学基金(2010011048-1);山西医科大学科技创新基金(01200806)资助项目

摘　　要：以胃癌细胞系BGC-823为研究对象,采用倒置相差显微镜观察纳米TiO2光催化氧化前后BGC-823细胞形态的变化,通过流式细胞仪考察其对BGC-823细胞凋亡率及细胞周期的影响。结果表明,纳米TiO2光催化氧化使BGC-823细胞呈受激坏死或凋亡晚期继发性坏死状态;导致BGC-823细胞周期阻滞于G2/M期并诱导细胞凋亡,从而抑制其DNA合成及增殖分化进程。当TiO2比负载量为9.8×10-7g/cm2、光照时间为40 min时,G2/M期阻滞较为显著,细胞凋亡率达24.34%。In order to explore the mechanisms of killing cancer cells via nano-TiO2 photocatalytic oxidization, gastric cancer cell line BGC-823 was cultured as research object in this study. The morphologic changes were observed by inverted phase-contrast microscope, the apoptotic rates and cell cycle of BGC-823 cells were examined by flow cytometer. The results showed that nano-TiO2-photocatalytic oxidation caused the necrosis or secondary necrosis of BGC-823 cells, and inhibited the synthesis of DNA and their proliferation process, through arresting the GJM phase and inducing apoptosis of BGC-823 cells. Significant arrest was observed arid the apoptotic rates of BGC-823 cells reached 24. 34% when the coated amount of TiO2 was 9. 8 × 10^ -7 g/cm^2 and the irradiation time was 40 min.

关 键 词：光催化氧化 纳米TIO2 胃癌细胞BGC-823 细胞周期 细胞凋亡 

分 类 号：O643[理学—物理化学] R735.2[理学—化学]