CdTe/CdS量子点与丝裂霉素的相互作用及其应用  

A Study on the Interactions Between CdTe/CdS Quantum Dots with Mitomycin C and Its Analytical Application

作　　者：李萍萍[1] 殷鹏飞[1] 龚会平[1] 刘正清[1] 王晓丹[1] 何佑秋[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《应用化学》2012年第6期705-710,共6页Chinese Journal of Applied Chemistry

基　　金：西南大学发光与实时分析重庆市重点实验室资助项目(CSTC;2006CA8006)

摘　　要：在水相中合成了硫普罗宁(Tiopronin,TP)修饰的CdTe/CdS量子点(TP-CdTe/CdS QDs)。利用紫外-可见吸收光谱、荧光光谱研究了TP-CdTe/CdS QDs与丝裂霉素(mitomycin C,MMC)的相互作用机理。在pH=7.6的tris-HCl缓冲溶液介质中,TP-CdTe/CdS QDs与MMC相互作用,使TP-CdTe/CdS QDs的荧光发生猝灭,并且QDs的荧光强度与MMC的浓度有良好的线性关系(r=0.999 1),线性范围4.7×10-9～1.2×10-8g/mL,检出限(3σ)为1.4×10-8g/mL。此方法快速简便,用于尿样中丝裂霉素的测定,实验结果令人满意。Tiopronin （TP） capped with CdTe/CdS quantum dots （TP-CdTe/CdS QDs） was synthesized in aqueous solution. The paper utilized ultraviolet-visible （UV-Vis） absorption spectra and fluorescence spectrometry to study the interactions between TP-CdTe/CdS QDs and mitomycin C （MMC）. With the optimum reaction conditions which was found to be at pH = 7.6 with tris-HC1 buffer solution, the quenched fluorescence intensity of TP-CdTe/CdS QDs was linearly proportional with the concentration of MMC, correlation coefficient r =0. 9991, and the detection limits were in the range of 4. 7×10^-9 - 1.2 × 10^-8 g/mL, and the detection limit（30-） for MMC was 1.4 × 10^-8g/mL. The quantitative determination of MMC is quick, simple and convenient. This method has been applied to the determination of MMC in urine samples with satisfactory results.

关 键 词：CDTE/CDS量子点 紫外-可见吸收光谱 荧光猝灭法 丝裂霉素 

分 类 号：O657.3[理学—分析化学]