我国棉花黄萎病菌致病力分化及ISSR指纹分析  

Pathogenicity differentiation and ISSR fingerprint analysis of cotton Verticillium dahliae in China

作　　者：朱荷琴[1,2] 冯自力[2] 尹志新[2] 李志芳[2] 赵丽红[2] 师勇强[2] 杨家荣[1] 

机构地区：[1]西北农林科技大学植物保护学院/作物逆境生物学国家重点实验室,杨凌712100 [2]国家棉花生物学重点实验室/中国农业科学院棉花研究所,安阳455000

出　　处：《植物病理学报》2012年第3期225-235,共11页Acta Phytopathologica Sinica

基　　金：国家公益性农业科研专项(3-19);转基因生物新品种培育重大专项课题(2009ZX08011-26B;2008ZX08011-002)

摘　　要：来自我国12个省84个县(市)的棉花黄萎病菌,在PDA培养基上存在5种不同的培养类型,其中,产生微菌核较多的B型菌株为优势类群,占72.9%。长江流域的菌株培养性状变异最大,新疆棉区的变异最小。致病力测定结果和ISSR指纹图谱均将167个单孢菌株划分为强、弱、中3个致病力类型,供试菌株的ISSR指纹图谱与菌株的致病力存在明显的相关性。中等致病力类型菌株在我国占主导地位;强致病力类型的菌株主要分布在河北、河南、湖北等省;弱致病力类型菌株主要分布在新疆和江苏。According to cultural characteristics on potato dextrose agar （PDA）, there were five different culture types （A to E） of Verticillium dahliae isolates from 84 counties of 12 provinces in China. Among them, Type B was the dominant group accounted for 72.9%, which produced abundant microslerotia on PDA. The isolates from Yangtze River region showed higher variation than those from Yellow River and those from Xinjiang varied slightly. Based on the results from the pathogenicity tests and ISSR fingerprint, 167 isolates were clustered into three groups which showed high, moderate and low virulence to cotton, respectively. There was a close co-relation between virulence and the ISSR fingerprint. Isolates with moderate virulence were dominant in China. High virulent isolates mainly distributed in Hebei, Henan and Hubei, whereas low virulent isolates mainly distributed in Xinjiang and Jiangsu.

关 键 词：棉花 黄萎病菌 致病力 ISSR 

分 类 号：S432.4[农业科学—植物病理学]