调肝与健脾对节育器致人子宫异常出血症子宫内膜组织ERK1/2、PI_3K信号通路的影响差异  

Effects of ERK1/2,and PI3K Signal Pathway of Endometrium Tissues with IUD Treated by Method of Regulating Liver and Strengthening Spleen

作　　者：刘秀华[1] 徐薇[2] 陈钢[1] 崔笛[1] 唐朋利[1] 朱蔓佳[1] 

机构地区：[1]成都中医药大学基础医学院,四川成都610075 [2]南方医科大学中医药学院,广东广州510515

出　　处：《辽宁中医杂志》2012年第5期777-778,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30171134;30772703)

摘　　要：目的:探讨柴胡止血液对置器后子宫异常出血患者的影响机制。方法:原代培养置器后子宫异常出血患者的宫内膜组织,给含药血清后,分别采用western blot和ELISA的方法测定ERK1/2和PI3K磷酸化的水平。结果:4号组织的归脾汤组升高了PI3K磷酸化水平,P<0.05;1号组织的柴胡止血液组有升高ERK1/2磷酸化水平的趋势;3号组织的柴胡止血液组有升高ERK1/2磷酸化水平的趋势;5号组织的柴胡止血液组有升高PI3K磷酸化水平的趋势;2号组织的归脾汤组有升高ERK1/2磷酸化水平的趋势;4号组织的归脾汤组有升高ERK1/2和PI3K磷酸化水平的趋势。结论:对于出血量大的肝旺患者,柴胡止血液可能通过激活ERK1/2通路来增强血管的收缩从而达到止血的目的;对于出血量较小的肝旺患者证,柴止血液可能通过激活PI3K通路来调节组织抗炎作用,从而达到治疗效果。Objective：To investigate the mechanisms of Chaihu Zhixue Liquid stopping bleeding to treat the uterus bleeding side-effect with IUD.Methods：Primary cultured the endometrial tissue,then treated them with drug-containing serum,using western blot and ELISA method to check the level of phosphorylation of ERK1/2 and PI3 K signal pathway.Results：For No.4 tissue,Guipi Decoction group increased the phosphorylation level of PI3K,P0.05.Conclusion：For large amount of bleeding of patients with liver excess,Chaihu Zhixu Liquid can activate ERK1/2 signal pathway to stop bleeding;for little amount of bleeding,Chaihu Zhixu Liquid can activate PI3K signal pathway to stop bleeding.

关 键 词：柴胡止血液 IUD ERK1/2 PI3K 

分 类 号：R203[医药卫生—中医学]