高效液相色谱法测定乳疾灵片中淫羊藿苷的含量  

Determination of Icariin in Rujiling Tablets by HPLC

作　　者：明延波 孟祥军[2] 吕洁 

机构地区：[1]辽宁卫生职业技术学院,辽宁沈阳110101 [2]沈阳医学院,辽宁沈阳110034

出　　处：《辽宁中医杂志》2012年第5期895-896,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:测定乳疾灵片中淫羊藿苷的含量。方法:采用HPLC法,以十八烷基硅烷键合硅胶为色谱柱:HypersilC18(250mm×4.6mm,5μm);流动相:0.075mol/L磷酸溶液(用三乙胺调节pH至4.5)-乙腈(73∶27);流速:1.0mL/min;检测波长:270nm。结果:淫羊藿苷在28.0～40.0μg/mL范围内,浓度与峰面积线性关系良好(r=0.9994)。平均回收率为100.84%,RSD为1.32%(n=9),方法的精密度、稳定性及重现性良好。结论:分析方法简便,快速准确,可作为乳疾灵片的质控标准。Objective：To determine icariin in Rujiling Tablets by HPLC. Methods： HPLC method was used： hypersil C18 （ 250mm×4.6 mm, 5μm） ; mobile phase ：0.075 mol / L phosphate solution （ pH = 4.5 ） - acetonitrile （73： 27 ） ; flow rate ： 1. 0mL/ min;detection wavelength：270nm. Results：The concentration and peak area of icariin had a good linear relationship（ r = 0. 9994） in 28. 0 - 40.0μg/mL. The average recovery was 100.84%, RSD was 1.32% （n = 9）. The method was precious and had stability and good reproducibility. Conclusion ：The analysis method is simple ,fast and accurate. Ruji Tablets can be used as quality control standards.

关 键 词：乳疾灵片 淫羊藿苷 高效液相色谱法(HPLC) 

分 类 号：R284[医药卫生—中药学]