罗布麻叶总黄酮抗抑郁作用参与5-HT能系统可能机制的研究  

Studies on the Antidepressant-Like Effect and 5-HT Mechanisms of Flavonoids Extracted from Apocynum venetum Leaves

作　　者：郑梅竹[1] 范亚军[2] 修瑾[3] 时东方[1] 刘春明[1] 

机构地区：[1]长春师范学院中心实验室,吉林长春130032 [2]长春师范学院生命科学院,吉林长春130032 [3]东北师范大学附属中学,吉林长春130022

出　　处：《辽宁中医杂志》2012年第5期935-937,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30970299);吉林省技厅重点项目(20090936);吉林省级教育厅资助项目([2012]D228)

摘　　要：该研究采用经典抗抑郁模型强迫游泳实验对罗布麻叶总黄酮的抗抑郁活性进行了评价,同时选择相关5-HT受体阻断剂(5-HT1A→Way100635、5-HT2→赛庚啶、5-HT2A→酮舍林)采用小鼠悬尾实验来探讨罗布麻叶总黄酮的抗抑郁机制。结果表明罗布麻叶总黄酮具有明确的抗抑郁作用;同时,5-HT2A、5-HT2受体阻断剂酮舍林(5mg/kg)、赛庚啶(3mg/kg)与罗布麻叶总黄酮50mg/kg联合连续灌胃给药10天后与罗布麻叶总黄酮50mg/kg单独给药相比,能明显延长悬尾小鼠累计不动时间(P<0.05),表明罗布麻叶总黄酮的抗抑郁活性与5-羟色胺能系统(5-HT2A、5-HT2受体)有关。In this study, the classical animal models of depression including the force swimming of the mice was applied to evaluate the antidepressant-like effect of AV-extract. To further search for the neurotransmitter mechanism of antidepression of AV- extract, additionally, the monoaminergic mechanisms involved in the antidepressant-like effect of AV-extract in mice were also as- sessed. The result showed that AV-extraet showed a clear antidepressant-like effect in variety of animal models of depression. The anti-immobility effect of AV-extraet（50mg/kg, p. o. ）was completely prevented by the pretreatment of mice with ketanserin（Smg/ kg, i. g. , a serotonin 5-HT2A receptor antagonist） , cyproheptadine （ 3 mg/kg, i. g. , a serotonin 5-HT2 receptor antagonist ）. On the other hand, the pretreatment of mice with Way 100635 （0.1 mg/kg, s. c. , a serotonin 5-HT1A receptor antagonist） , did not block the antidepressant-like effect of AV-extract in the TST. Taken together, the data demonstrated that AV-extraet produced an antide- pressant-like that seems to be dependent on its interaction with the serotonergie（5-HT2nand 5-HT2 receptors）systems.

关 键 词：罗布麻 抗抑郁 机制 

分 类 号：R285.5[医药卫生—中药学]