亚低温对肝缺血再灌注损伤的保护作用  

Protective effect of mild hypothermia on liver injury of hepatic ischemia-reperfusion

作　　者：何劲松[1] 姜立华[1] 杨新[1] 吕新生[2] 徐明清[1] 赖家骏[1] 杨献群[1] 

机构地区：[1]广东省粤北人民医院普外科,广东韶关512026 [2]湖南医科大学附属湘雅医院普外科,湖南长沙410008

出　　处：《中国普通外科杂志》2000年第2期132-134,共3页China Journal of General Surgery

摘　　要：目的　探讨亚低温对肝缺血再灌注损伤的保护作用机制。方法　将 18只犬随机分为 3组 :非缺血对照组 (n =6 )、缺血再灌注组 (n =6 )和亚低温处理组 (肝周充填碎冰块造成肝脏亚低温 ,n =6 )。对各组肝上下腔静脉血进行谷丙转氨酶 (ALT)、谷草转氨酶(AST)、乳酸脱氢酶 (LHD )以及丙二醛 (MDA)和超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化酶 (GSH PX)活性及总抗氧化 (TAX )能力测定。结果　全肝缺血再灌注后ALT ,AST ,LDH和MDA含量明显上升 (P <0 .0 1) ,SOD ,CAT ,GSH PX活性及TAX能力明显下降 (P <0 .0 1) ;而亚低温处理组与缺血再灌注组比较 ,ALT ,AST ,LDH和MDA含量明显下降 (P <0 .0 1) ,SOD ,CAT ,GSH PX活性及TAX能力明显上升 (P <0 .0 1,P <0 .0 5 )。结论　亚低温能增强肝组织自身抗氧化能力 ,减轻肝缺血再灌注后氧自由基对肝脏的损伤。Objective To study the mechanisms of protective effect of mild hypothermia on hepatic ischemia reperfusion(I R) injury. Methods Eighteen dogs were randomly divided into three groups: nonischemic control(C) group(n=6), ischemia reperfusion(I R) group(n=6) and mild hypothermia treatment(MHTT) group(n=6). The measurement were taken in all the three groups for the content of the marker enzymes of liver damage(ALT,AST,LDH) and malondialdehyde(MDA), and activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GSH PX), total antioxidase(TAX) in inferior vena cava blood above liver. Results Aftre I R, the content of ALT, AST, LDH and MDA were significantly elevated(P<0.01), SOD, CAT, GSH PX, ACT activeties declined obviously(P<0.01), but the content of ALT, AST, LDH and MDA obviously lowered in MHTT group than that in I R group(P<0.01); and the content of SOD, CAT, GSH PX, ACT activeties were obviously higher than that in I R group(P<0.01, P<0.05). Conclusions Mild hypothermia may enhance liver ability of oxidation resistance, and reduce the oxygen free radical injury to liver after ischemia reperfusion.

关 键 词：再灌注损伤/预防和控制 低温 人工 肝功能减轻 疾病模型 动物 

分 类 号：R657.3[医药卫生—外科学]