Ca^(2+)对皖贝母高温胁迫下抗逆生理指标及光合作用的影响  

Effects of exogenous Ca^(2+) on physiological and photosynthesis of Fritillaria anhuiensis under high temperature stress

作　　者：李同根[1] 王康才[1] 罗庆云[1] 李丹霞[1] 

机构地区：[1]南京农业大学中药材研究所,南京210095

出　　处：《植物营养与肥料学报》2012年第3期765-770,共6页Journal of Plant Nutrition and Fertilizers

基　　金：深圳市三九现代中药有限公司项目(2010126)资助

摘　　要：分析Ca2+对高温胁迫下皖贝母抗逆生理指标及相关光合参数的影响,探讨钙对皖贝母高温胁迫伤害调控的可行性。将室外同一环境条件下盆栽的皖贝母,分别叶面喷施5、10、20、30、50 mg/L的CaCl2溶液,然后置于35℃/25℃(昼/夜),光强3600 lx的光照培养箱内,进行高温胁迫处理,以常温25℃喷施蒸馏水和高温胁迫下喷施蒸馏水为对照,处理3 d后,测定皖贝母叶片相关光合参数、膜透性、脯氨酸含量、可溶性蛋白含量及SOD、POD活性。结果表明,高温胁迫下叶面喷施CaCl2溶液,可增强皖贝母叶片SOD、POD酶活性,提高叶片游离脯氨酸及可溶性蛋白含量,有效减少高温胁迫对细胞膜的破坏,显著降低相对电导率。同时,Ca2+处理提高了叶片中叶绿素与类胡萝卜素含量,提高了叶片光合效率。叶面喷施一定浓度CaCl2溶液可减轻高温胁迫对皖贝母叶片的伤害。In order to reveal feasibility of the regulation of Ca^2＋ on Fritillaria anhuiensis under high temperature stress, the effects of Ca^2＋ on physiological index and related photosynthetic parameters were studied. Pot cultured Fritillaria anhuiensis leaves under the same outdoor conditions were sprayed with 5, 10, 20, 30 and 50 mg/L CaC12 separately, and then placed in the light incubator [ 35℃/25℃ （day/night）, light intensity 3 600 lx ], and sprayed with distilled water at 25℃ and under high temperature stress were set as the control. After treated for 3 days, related photosynthesis, relative conductivity, contents of proline and soluble protein, activities of SOD and POD were tested. The results show that the exogenous GaS~ enhances the activities of SOD and POD, the contents of proline and soluble protein, and effectively reduces the damage of heat stress on cell membrance. At the same time, the exogenous Ca2 ＋ increases the contents of chlorophyll and carotenoid, the efficiency of leaf photosynthesis. In conclusion, the foliage spraying CaC12 could reduce the damage of high temperature stress on Fritillaria anhuiensis leaves.

关 键 词：皖贝母 CA2+ 高温胁迫 光合作用 

分 类 号：S567.231[农业科学—中草药栽培]