气相色谱法检测桑叶中有机氯及菊酯类农药  

Determination of Organochlorine and Pyrethrpid Pesticides in Mulberry Leaf by Gas Chromatography

作　　者：黄晓会[1,2] 薛健[1] 吴晓波[1] 佟鹤芳[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100193 [2]宁夏医科大学研究生学院,宁夏银川750004

出　　处：《分析科学学报》2012年第3期328-332,共5页Journal of Analytical Science

基　　金：国家重大创新药专项(No.2009ZX09502-027;2009ZX09308-006;2009ZX09308-001-1-5)

摘　　要：建立了毛细管气相色谱法检测桑叶中农药多残留的方法。样品以乙腈为提取剂,提取液用弗罗里硅土柱净化,乙酸乙酯∶正己烷(体积比15∶85)洗脱,采用HP-5与DB1701毛细管色谱柱双柱,气相色谱-电子捕获检测法(GC-ECD)测定42种有机氯及拟除虫菊酯类农药及其异构体的残留量。结果表明:该方法在20、80、160μg/kg 3个添加水平下回收率分别为78.6%～110.3%、78.3%～109.6%、80.1%～109.1%,相对标准偏差(RSD)均在1.1%～11.3%,符合农药残留分析的要求。该方法重复性及净化效果好,可用于桑叶中42种有机氯及拟除虫菊酯类农药及其异构体的残留量检测。A gas chromatography method for the determination of pesticides multi-residues in mulberry leaf was established.The organochlorine and pyrethroid pesticides were extracted from mulberry leaf with petroleum acetonitrile.Then the extracts were cleaned up by alumina neutral-florisil column and eluted by mixed solvents of ethyl acetate and hexane（15∶85,V/V）.The extracts were separated by HP-5 and DB1701dual capillary column and detected by electron-capture detector.Results show that the recoveries were from 78.6%to 110.3%,from 78.3%to 109.6%and from 80.1%to 109.1% with RSD ranged from 1.1%to 13.1%,at three spiked mixed organochlorine and pyrethroid levels,respectively.The method has good separation ability and repeatability and can be used in determination of organochlorine and pyrethroid pesticide residues in Mulberryleaf products.

关 键 词：桑叶 毛细管气相色谱 有机氯农药 拟除虫菊酯农药 

分 类 号：O657.7[理学—分析化学]