普萘洛尔对体外培养的血管瘤内皮细胞凋亡的影响  

Effects of Propranolol on Apoptosis of Hemangioma Endothelial Cells in Vitro Culture

作　　者：魏珩[1] 俞松[1] 姜富贵[1] 李堂江[1] 谢祎[1] 罗宇[1] 彭刚[1] 徐艳朋[1] 

机构地区：[1]遵义医学院附属医院小儿矫形外科,贵州遵义563003

出　　处：《实用儿科临床杂志》2012年第11期821-822,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81160330);贵州省省长资金项目[黔省专合字(2010)67号];遵义市重点科技计划项目培育及人才培养计划项目[遵科培字(2009)04号]

摘　　要：目的观察普萘洛尔对体外培养的血管瘤内皮细胞凋亡的影响。方法取婴幼儿增殖期血管瘤标本,用组织块法原代培养血管瘤内皮细胞,并用第Ⅷ凝血因子相关抗原进行鉴定。待细胞处于对数生长期,换无血清培养基孵育48 h,使细胞同步化,然后分别加入0.5μmol·L-1和1.0μmol·L-1的普萘洛尔作为干预组,而对照组不加任何干预因素,继续孵育24 h,检测血管瘤血管内皮细胞凋亡率。结果血管瘤内皮细胞于组织块接种后3～4 d开始贴壁生长,内皮细胞呈多角形态,3～4周细胞开始快速生长,并铺满板底。培养细胞第Ⅷ凝血因子相关抗原表达为阳性。普萘洛尔作用24 h后,0.5μmol·L-1普萘洛尔组和1.0μmol·L-1普萘洛尔组血管瘤内皮细胞凋亡率分别为(22.42±0.83)%、(24.36±1.42)%,较对照组[(15.72±0.59)%]均明显升高(Pa<0.01)。结论普萘洛尔可以促进体外培养的血管瘤内皮细胞凋亡或抑制其增殖。Abstract ： Objective To observe the influence of propranolol on the apoptosis of hemangioma endothelial cells in vitro culture. Methods The hemangioma vascular endothelial cells which came from the specimens of infant with hemangioma were cultured by explant combined with trypsin - digested technique. The expression of the factor V~ related antigen which only existed in endothelial cells to identify the heman- gioma vascular endothelial cell studied by the immunohistochemistry technique. When the cells were in the logarithmic phase,the culture me- dium was exchanged to serum -free medium to make the cell synchronization in 48 hours. Then,the specimens were incubated with different concentrations of propranolol（0.5 μmol ·L -1 and 1.0 μmol · L-1 ） as intervention group,and the control group was not given any interven- tion. The cell apoptosis were investigated by flow cytometry technique after 24 hours. Results The growth of hemangimna endothelial cells which took a polygonal could be seen around the tissue fragments adhering to the wall after 3 - 4 days, and began to grow fast and covered the bottom of culture bottles after three or four weeks. The expression of factor Ⅷ associated antigen was positive in the endothelial cells in vitro culture. The apoptosis rate of hemangioma endothelial ceils was （ 22.42 ＋ 0.83 ） % in 0.5 pμmol · L- 1 propranolol group and （ 24.36 ＋ 1.42） % in 1.0 μmol ·L t propranolol group, both of them were significantly higher than that of the control group[ （15.72 s-0.59 ）% ] （Pa 〈 0.01 ）. Conclusion Propranolol may promote the apoptosis or inhibit the growth of hemangioma vascular endothelial cells in vitro culture.

关 键 词：血管瘤 血管内皮细胞 细胞培养 普萘洛尔 凋亡 

分 类 号：R725.4[医药卫生—儿科]