促红细胞生成素对急性下肢缺血性疾病大鼠模型的治疗作用  

Effect of erythropoietin on experimental rats with acuteischemic disease in lower limbs

作　　者：亢鑫[1] 杨涛[2,3] 曹文东[2,3] 皮兴涛[2,3] 续慧民[2,3] 郝斌[2,3] 

机构地区：[1]山西医科大学研究生院,山西太原030001 [2]山西医学科学院 [3]山西大医院血管外科,山西太原030001

出　　处：《中国现代医生》2012年第14期3-5,共3页China Modern Doctor

摘　　要：目的探讨促红细胞生成素(EPO)对大鼠急性下肢缺血性疾病的保护作用。方法将急性下肢缺血性疾病大鼠模型分为生理盐水组和EPO组,按缺血时间不同分为6 h、1 d、2 d、3 d组,采用RT-PCR方法观察HIF-1α变化规律,免疫组化法检测MVD的计数情况。结果通过各时间点比较,EPO组HIF-1α均低于生理盐水组,而MVD均高于生理盐水组,差异有统计学意义(P<0.05)。结论早期应用EPO能明显降低急性下肢缺血大鼠的HIF-1α、提高MVD,对治疗急性下肢缺血性疾病具有重要意义。Objective To observe the the effects of erythropoietin on the expression of hypoxia inducible factor-lot and microvascular density in acute limb ischemia as well as investigate the protection effect of erythropoietin. Methods The ex- perimental rats with acute limb ischemia are separated into two groups： one was injected with 1000 U/kg EPO and the other was injected with 1000 U/kg normal saline. Each group was diyided into 4 subunits by the time of ischemia： 6 hours, 1 day, 2 days and 3 days. The changes of HIF-1α intensity were observed through RT-PCR and MVD counting state was tested through immunohistochemistry. Results The concentration of HIF-1α in rats injected with EPO was lower than the ones injected with normal saline in every subunit and the difference was notable （P 〈 0.05）. MVD counting state in rats injected with EPO was higher than the ones injected with NaCL and the difference was notable（P 〈 0.05）. Conclusion In the pro- cess of treating acute limb ischemia, injection of EPO in the first few days has obvious therapeutical effect on experimental rats through reducing HIF-1α and improving MVD.

关 键 词：急性下肢缺血性疾病 促红细胞生成素 缺氧诱导因子 微血管密度 

分 类 号：R654.3[医药卫生—外科学]