花粉多糖的分离纯化及其组成单糖分析  

Isolation and Purification of Polysacchrides from Pollen of Rap and Analysis of Their Components

作　　者：任金山[1] 吴梧桐[1] 李颖[2] 安雪飞[1] 李莉[1] 

机构地区：[1]中国药科大学生物化学教研室 [2]中国药科大学分析计算中心

出　　处：《中国药科大学学报》1990年第3期173-175,共3页Journal of China Pharmaceutical University

摘　　要：油菜花粉经破壁、热水提取,用三氯醋酸除杂蛋白、透析、乙醇沉淀获得粗品多糖。用CTAB络合法分离了花粉酸性多糖与中性多糖成分。中性多糖用Sephadex G-100凝胶柱层析进一步纯化得P-A、P-B、P-C 3个组分。酸性多糖用DEAE-Sephadex A-25纯化得P-D、P-E 2个组分,聚丙烯酰胺凝胶电泳均为单一区带。P-A、P-B、P-C、P-D、P-E的酸水解产物经气相色谱分析表明,均含L-岩藻糖、L-阿拉伯糖、D-木糖、D-甘露糖、D-半乳糖、D-葡萄糖及L-鼠李糖。而酸性多糖除含以上单糖组分外,还含有已糖醛酸,但不含硫酸基。The broken pollen of rap (BPR) was extracted by boiling water. The protein in the extract was removed by trichoroacetic acid, and BPR was dialysised. The crude polysacchrides (CP) were precipitated by alcohol and acetone. The CP could improve dramatically immunological function. The both acidic and neutural polysacchrides were botained from CP. Three pure polysacchrides were obtained from neutural polysacchrides by Sephadex G-100 chromatography and named P-A, P-B and P-C. Two pure polysacchrides were obtained from acidic polysacchrides by Sephadex A-25 chromatography and named P-D and P-E. The components of those pure polysacchrides were hydrolysed and identified by gas chromatography. P-A, P-B, P-C, P-D and P-E are composed of L-fucose, L-arabinose, D-xylose, D-mannose, D-galactose, D-glucose and L-rhammose. Both P-D and P-E still contain hexuronic acid, but no sulfate group.

关 键 词：花粉 多糖 分离 分析 纯化 

分 类 号：R284.2[医药卫生—中药学]