盐胁迫下外生菌根菌Paxillus抗氧化酶活性的变化  

Changes of Anti-oxidative Enzymes Activity of Paxillus involutus under Salt Stress

作　　者：包斯琴[1] 李静[1] 孙健[1] 马旭君[1] 陈少良[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《安徽农业科学》2012年第15期8401-8403,8405,共4页Journal of Anhui Agricultural Sciences

基　　金：北京市优秀博士学位论文指导教师专项基金(YB20081002201);教育部重点项目(209084);国家自然科学基金项目(30872005);北京市自然科学基金项目(6112017);中央高校基本科研业务费专项资金(JC2011-2)

摘　　要：[目的]通过测定2种菌株的抗氧化酶活性来研究其抗盐机理。[方法]以外生菌根真菌Paxillus involutus的2种菌株MAJ和NAU作为材料,研究在不同NaCl浓度(0、100、200、500 mmol/L)处理条件下抗氧化酶活性的变化。[结果]100 mmol/L NaCl可以促进菌株MAJ和NAU的增长及其生物量的增加;短期盐胁迫(1 d)诱导了菌株MAJ中SOD、CAT和POD活性的升高,但随着盐处理时间的延长,这些酶活性在100和200 mmol/L盐胁迫下均有所降低;而菌株NAU在长期盐胁迫(9 d)下能更好地维持各抗氧化酶活性,尤其是APX、GPX和POD。[结论]盐处理的菌株抗氧化酶活性明显高于对照菌株。[ Objective ] Salt resistance mechanism of two strains of antioxidant enzymes was studied through the determination of their activity. [ Method ] The effects of different concentration of NaCl on antioxidative enzymes activity （SOD, CAT, APX, POD and GPX） of Paxillus involutus （strains MAJ and NAU） were investigated. [ Result] The growth and biomass of the two strains MAJ and NAU were both improved under 100 mmol/L NaCl treatment. Activities of anti-oxidative enzymes were increased in strain MAJ after a short-term salinity （ 1 d）. However, the activity of antl-oxidative enzymes declined with the increase of NaCl concentrations and the duration of salt treatment. Under a long-term salinity （9 d）, strain NAU remained the activities of anti-oxidative enzymes, especially APX, GPX and POD. [ Conclusion ] Anti-oxidantive enzymes activity of salt treatment were higher than those of control evidently.

关 键 词：Paxillus involutus MAJ NAU 抗氧化酶活性 耐盐性 

分 类 号：S156.4[农业科学—土壤学]