应用特异PCR方法诊断2例猪弓形虫病  

Diagnosis of Swine Toxoplasmosis by PCR Assay

作　　者：黄翠琴[1,2] 陈永锋 王寿昆[4] 郑新添[1,5] 李海丽[1] 

机构地区：[1]龙岩学院生命科学学院,福建龙岩364000 [2]福建省人畜寄生与病毒性疫病防控工程技术研究中心,福建龙岩364000 [3]厦门市农产品质量安全检验测试中心,福建厦门361000 [4]福建农林大学动物科学学院,福建福州350001 [5]预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364000

出　　处：《安徽农业科学》2012年第15期8553-8554,8557,共3页Journal of Anhui Agricultural Sciences

基　　金：福建省科技厅福建省属高校科研项目(JK2011052);福建省科技计划项目(2008N2005);福建省高等学校杰出青年科研人才培育计划项目(2011)

摘　　要：[目的]应用特异PCR方法诊断猪弓形虫病。[方法]分别以弓形虫核糖体DNA第一内部转录间隔区(ITS1)序列和529 bp重复序列为目的片段,扩增2例疑似猪弓形虫病的病料肺门淋巴结DNA,对疑似猪弓形虫病的病料进行PCR诊断。[结果]2对引物均能分别扩增出2例肺门淋巴结中弓形虫DNA的特异条带。[结论]基于2种扩增片段的PCR方法均具有较高的特异性,是诊断猪弓形虫病的一种快速检测方法。[ Objective ] The aim was to diagnose swine toxoplasmosis by PCR assay. [ Method ] Two cases of suspected swine toxoplasmosis from different farms were collected by clinical diagnosis. The hilar lymph node DNA of the two cases were extracted and the first internal transcribed spacer of ribosomal DNA （ ITS1 ） and the repetitive 529 bp fragment were amplified by PCR assay using two pairs of primers, respectively. [ Result] The DNA fragments specific for Toxoplasma gondii were both amplified in ITS1 and the repetitive 529 bp fragment from infected tissues genomic DNA in both cases by PCR assay, respectively. [ Conclusion] Based on two genetic markers, PCR assay is a rapid and accurate method for diagnosing swine toxoplasmosis with high specificity.

关 键 词：猪 弓形虫病 PCR 诊断 

分 类 号：S828[农业科学—畜牧学]