白纹伊蚊实验种群感染沃尔巴体Wolbachia的研究  

STUDY ON WOLBACHIA INFECTION IN EXPERIMENTAL POPULATION OF AEDES ALBOPICTUS

作　　者：张东京[1] 杨潇[1] 吴贤生[1] 陈婧[1] 李卓雅[1] 吴瑜[1] 詹希美[1] 郑小英[1] 

机构地区：[1]中山大学中山医学院寄生虫教研室,中山大学热带病研究教育部重点实验室,广州510080

出　　处：《寄生虫与医学昆虫学报》2012年第1期12-18,共7页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：为了了解白纹伊蚊实验种群沃尔巴体Wolbachia感染率、感染品系、组织分布及系统发育,运用引物PCR方法检测实验种群白纹伊蚊Wolbachia 的感染情况.解剖白纹伊蚊,提取头部、卵巢/睾丸、脂肪体、唾液腺/胸部、马氏管、中肠组织的基因组DNA,PCR法扩增Wolbachia表面膜蛋白（wsp）,扩增产物进行克隆及测序,并用BLAST软件对其进行系统发育分析.将随机抽取的白纹伊蚊雌雄各50只分别检测,均为阳性.卵巢/睾丸、脂肪体和唾液腺/胸部均存在着Wolbachia A、B组超感染;雌蚊头部、雄蚊马氏管和中肠为B组单感染;雄蚊头部则未检测到Wolbachia.将wsp基因克隆测序后进行系统发育分析显示白纹伊蚊体内Wolbachia与尖音库蚊、菜蛾、宽边黄粉蝶四者同源性较高达99%,这表明四者体内所感染的Wolbachia可能来源于同一个分支.In order to investigate the infection profiles of Wolbachia in Aedes albopictus rearing in laboratory, we carried out a survey in Ae. albopictus using polymerase chain reaction （PCR） assay to detect Wolbachia to analysis the infection rates, strains, tissue distribution and the phylogeny relationships. Briefly, template DNA was extracted from the head, ovary/ testis, fat body, salivary glands/thorax, malpighian tubules and midgut respectively to amplify Wolbachia Surface Protein （wsp） gene. The amplification product was cloned and sequenced to analyze the phylogenesis with BLAST tools. Of fifty male and female mosquitoes randomly selected, all of them were positively infected with Wolbachia. The ovary/tesis, fat body and salivary glands/thorax of both sexes were superinfected by Wolbachia A and B strains. The malpighian tubules and midgut of male were only infected by B strain. As for the head, the female were found only infected by B strain, the male were not found infection. The identity of Wolbachia in Ae. albopictus, is 99% as high as Culex pipents, Cotesia sesamiae and Eurema necabe, which suggests that Wolbachia in Ae. albopictus, Culex pipents, Cotesia sesamiae and Eurema necabe may originate from the same subgroup.

关 键 词：沃尔巴体Wolbachia 白纹伊蚊 表面膜蛋白(wsp) 系统发育 

分 类 号：Q969.442.2[生物学—昆虫学]