红景天甙抑制兔缺氧性肺动脉平滑肌细胞增殖的作用  

Inhibitory effects of salidroside on hypoxia induced proliferation of rabbit pulmonary arterial smooth muscle cells

作　　者：赵贺玲[1] 林树新[1] 贾斌[1] 李剑[1] 张莉莉[1] 张世范[2] 

机构地区：[1]第四军医大学基础部病理生理学教研室,陕西西安710033 [2]兰州军区总医院胸外科

出　　处：《第四军医大学学报》2000年第2期186-189,共4页Journal of the Fourth Military Medical University

摘　　要：目的　观察红景天甙 (salidroside,Sal)对缺氧状态下兔肺动脉平滑肌细胞 (PASMC)增殖、DNA合成和细胞周期的影响 ,并初步探讨其作用机制 .方法　应用离体培养的PASMC,采用四唑盐 (MTT)比色法实验、3H-胸腺嘧啶核苷(3H- Td R)参入和流式细胞仪技术 ,观察 PASMC的增殖、DNA合成和细胞周期的变化 .结果　缺氧 2 4h可直接刺激PASMC,使其3H- Td R的参入量显著增加 (P<0 .0 1 ) ,MTT吸光度 (A)增加 (P<0 .0 5 ) ;钙通道阻滞剂 verapamil可抑制缺氧的这种刺激作用 ;流式细胞仪分析显示缺氧 2 4h PASMC的 G2 /M期细胞比例与常氧对照组相比明显增加 (P<0 .0 1 ) ,G0 /G1 期细胞比例明显减少 ;在缺氧的 PASMC培养液中加入 Sal(0 .2～ 3.2 μmol· L- 1 ) ,PASMC的 3H- Td R参入量与 MTT的 A值与单纯缺氧组相比显著下降 ,Sal(6 .4μmol· L- 1 )可增加 G0 /G1 期细胞比例 ,减少 G2 /M期细胞比例 (P<0 .0 5 ) ;缺氧状态下 ,Sal与 verapamil合用并不增加对PASMC的抑制效应 .结论　缺氧可直接刺激 PASMC的增殖及 DNA合成 ,其作用机制可能与 PASMC内 Ca2 +浓度增加有关 ,Sal可抑制缺氧对 PASMC的增殖及AIM To investigate the effects of salidroside on the proliferation, the DNA synthesis and cell cycle of rabbit pulmonary arterial smooth muscle cell(PASMC)under hypoxia and to study its mechanism. METHODS Techniques of cell culture, MTT test, 3H TdR incorporation and flow cytometric DNA analysis were used. RESULTS PASMC 3H TdR incorporation and A value of MTT test increased significantly in response to 24 hour hypoxia culture, and verapamil (80 μmol·L -1 ), a Ca 2+ channel bloker, could inhibit the effect induced by hypoxia. When salidroside (0.2, 0.8, 3.2×10 μmol·L -1 ) were added into the medium, 3H TdR incorporation and OD value of MTT decreased significantly as compared with hypoxia group. Flow cytometric DNA analysis revealed that hypoxia significantly enhanced G2/M phase of PASMC and decreased G 0/G 1 phase of PASMC ( P <0.01). Salidroside (6.4 μmol·L -1 ) could reverse this effect ( P <0 05). But no further inhibitory effects were observed by co incubation of verapamil (80 μmol/l) and sal (3.2 μmol·L -1 ). CONCLUSION The results reveal that hypoxia can directly stimulate the proliferation and DNA synthesis, and its mechanism may be associated with the increase of intracellular [Ca 2+ ]. Salidroside can inhibit the enhancing effects of hypoxia on the proliferation and DNA synthesis of PASMC.

关 键 词：缺氧 肺动脉 平滑肌细胞增殖 红景天甙 

分 类 号：R285.5[医药卫生—中药学] R543.5[医药卫生—中医学]