MN9202保护血栓大鼠尾动脉血管作用  

MN9202 protection of tail artery in carrageenan induced thrombosis rats

作　　者：王莉莉[1] 李志超[1] 梅其炳[1] 赵德化[1] 

机构地区：[1]第四军医大学基础部药理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2000年第2期214-216,共3页Journal of the Fourth Military Medical University

基　　金：国家科委医药创新博士基金!资助项目 ( 94-B-0 4)

摘　　要：目的　观察新二氢吡啶类钙拮抗剂 2 ,6 -二甲基 - 4-(3-硝基苯基 ) - 1 ,4-二氢 - 3,5 -吡啶二羧酸 - 3-甲酯 - 5 -正戊酯(MN92 0 2 )对血栓形成大鼠尾动脉血管平滑肌及内皮功能的影响 .方法　大鼠脚趾 sc注射角叉菜胶制备血栓模型 ,并于注射角叉菜胶前 2 4h,1 h及注射后 2 4h ip MN92 0 2 1μmol·kg- 1 .取尾动脉血栓波及不同区域的三段血管 ,以去甲肾上腺素 (NE) ,乙酰胆碱 (ACh) ,亚硝酸钠 (Na NO2 )为诱导剂 ,进行离体血管条试验 .结果　与正常对照组比较 ,模型组尾动脉对3种诱导剂的反应性显著下降 ,由近端至远端逐渐增加 ,远端血管的舒张反应完全丧失 .与模型组比较 ,MN92 0 2组尾动脉各段对 NE的收缩反应 (mg)有所增强 ,尤以近端最为显著(1 35± 2 5 vs88± 2 0 ,P<0 .0 5 ) ;近端及中段尾动脉血管对ACh诱导的舒张反应显著改善 ,Emax分别为 (34± 3.8) % vs(2 7± 3.1 ) %和 (32± 3.4) % vs (2 4± 4.5 ) % (P<0 .0 5 ) ,对亚硝酸钠诱导的舒张反应有所增加 .结论　 MN92 0 2对尾血栓造成的尾动脉血管平滑肌及内皮损伤具有保护作用 。AIM To investigate the effects of methyl pentyl 1, 4 dihydro 2, 6 dimethyl 4 (3 nitrophenyl) 3, 5 pyridinedicarboxylate (MN9202) on vascular smooth muscle and endothelium cells of tail artery in thrombosis rats induced by carrageenan. METHODS A thrombosis model in rats tail was established by injecting carrageenan sc. in paw. MN9202 at dosage of 1 μmol·kg -1 , ip at 24 and 1 h before and 24 h after treatment with carrageenan. Isolated vascular experiment was performed in proximal, middle and distant sections of tail artery. RESULTS Compared with those of the control group, the responses of tail arterial strips to NE (norepinephrine), ACh (acetylcholine) and NaNO 2 decreased significantly and weaker from proximal section to distant sections. The vasorelaxation of distant strips induced by ACh and NaNO 2 was impaired completely. Compared with the model group, MN9202 partially attenuated the carrageenan induced inhibition of vasoconstriction (mg)(135±25 vs 88±20, P <0.05)and the endothelium dependent relaxation in proximal and middle section strips, Emax being (34±3.8)% vs (27±3.1)%, and (32±3.4)% vs (24±4.5)% ( P <0 05), respectively. In addition, endothelium independent relaxation was also improved by MN9202. CONCLUSION The anti thrombosis effects of MN9202 on thrombosis rats induced by carrageenan may be in part due to the protection of vascular smooth muscle and endothelium.

关 键 词：血栓形成 乙酰胆碱 MN9202 血管舒张 血管收缩 

分 类 号：R972.4[医药卫生—药品]