丙型肝炎实验室诊断方法及相关指标的探讨  

Laboratory diagnosis of hepatitis C and its related indicators

作　　者：袁乐永[1] 李艳[1] 徐万洲[1] 陈进[1] 吴泽刚[1] 

机构地区：[1]武汉大学人民医院检验科,湖北省武汉市430060

出　　处：《职业与健康》2012年第9期1086-1088,共3页Occupation and Health

摘　　要：目的探讨丙型肝炎病毒RNA(HCV-RNA)定量检测及抗丙型肝炎病毒(anti-HCV)检测在丙型肝炎病毒感染诊断中的价值,以及HCV感染对血脂代谢的影响。方法收集anti-HCV阳性丙型肝炎患者与正常健康人标本分别为40和84例,检测样本血浆中HCV-RNA,血清anti-HCV抗体、超敏C-反应蛋白(hs-CRP)、载脂蛋白AI(apoAl)、载脂蛋白B(apoB)、甘油三酯(TG)、总胆固醇(TC)、脂蛋白(a)[LP(a)]、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)与总胆红素(TBIL)等指标,并结合临床诊断对结果进行分析。结果 40例anti-HCV阳性患者中,HCV-RNA阳性率为66.7%,hs-CRP、apoAl、apoB、TG、TC、LP(a)、HDL-C、LDL-C、ALT、AST和TBIL的含量与正常健康人差异有统计学意义(P<0.05)。结论 anti-HVC和HCV-RNA的联合检测,对于丙型肝炎的诊断及治疗具有重要的实用价值及临床意义,如与生化等指标同时检测,将会对临床更有意义。[ Objective]To explore the value of quantitative detection of HCV-RNA and Anti-HCV detection in clinical diagnosis, and the effect of HCV infection on blood lipid metabolism. [ Methods] Anti-HCV positive hepatitis C patients specimens of 40 cases and normal healthy people specimens of 84 cases were collected respectively to detect HCV-RNA in plasma and test Anti-HCV, hs- CRP, apoAI, apoB, TG, TC, LP（a） , HDL-C, LDL-C, ALT, AST, and TBIL etc in serum, and the results were analyzed combi- ning with clinical diagnosis. [ Results] Of 40 cases of anti-HCV positive hepatitis C patients, the detection rates of HCV-RNA was 66.7%; the concentrations of hs-CRP, apoA1, apoB, TG TC. LP（ a I, HDL-C, LDL-C, ALT, AST, and TBIL were significantly different with those of the healthy people （ P 〈 0.05 ）. [ Conclusion] The combined detection of anti-HCV and HCV-RNA has prac- tical value and clinical significance in diagnosis and treatment of hepatitis C, especially with simultaneous detection of biochemical indicators

关 键 词：抗丙型肝炎病毒 丙型肝炎病毒RNA 超敏C-反应蛋白 血脂 

分 类 号：R446[医药卫生—诊断学]