慢性扁桃体炎患儿扁桃体抗隐窝上皮角蛋白的免疫组化观察  

Immunologic histochemical observation of anti- cryptkeratin of cryptic epithelium of tonsilla in children with chronic tonsillitis

作　　者：王智楠[1] 徐忠强[1] 王淑芬[1] 鲁建生[2] 冯亭[2] 王竞春[2] 

机构地区：[1]武汉市儿童医院耳鼻咽喉科,武汉430016 [2]武汉市儿童医院病理科

出　　处：《临床耳鼻咽喉科杂志》2000年第3期122-123,共2页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨慢性扁桃体炎患儿扁桃体隐窝上皮的变化。方法 :将摘除的 2 30例扁桃体均以 10 %福尔马林固定 ,常规石蜡包埋切片 ,HE染色 ,并行抗隐窝上皮角质蛋白 (CK)免疫组化检测 ,观察小儿慢性扁桃体炎症变化。结果 :2 30例扁桃体隐窝上皮明显角化 2 0 9例 (90 .9% ) ,未见角化 2 1例 (9.1% ) ;3例隐窝底部有霉菌菌丝和细菌 ;扁桃体隐窝上皮抗广谱和高分子 CK均呈阳性反应 ,全组隐窝内角化物抗广谱 CK亦均呈阳性反应。结论 :小儿扁桃体每次炎症病变 ,都使扁桃体上皮进行性破坏 ,扁桃体产生免疫球蛋白的物质减少 ,因而不能有效发挥保护作用 ,而细菌、病毒等物质却可顺利通过化生性上皮向上皮下侵犯 ,这种周而复始的恶性循环 。Objective:To investigate anti- crypt keratin (CK ) im munologic histochem ical changes in children with chronic tonsillitis.Method:Rem oved tonsilla were fixed by10 % formaldehyde.Imm unologic histochemical m ethod was used to determ ine the changes of anti- broad spectrum (KD6 8、5 6、5 6、5 0 ) CKSPTM .Result:In 2 30 cases,obvious keratosis was90 .9% ,no keratosis was 9.1% ,3cases were found with fungus filam ents and bacteria in the bottom of crypts.Anti- broad spectrum and hyperm olecule CK of tonsil cryptic epithelium were positive reaction,anti- broad spectrum CK of cryptic keratosis in all cases was positive reaction.Conclusion: During the period of episode,cryptic epitheliums of tonsilla was destroyed repeatly,therefore,im munoglobulin production was reduce.Because the im mune function of tonsilla was reduced,bacteria and virus m ight be invade into organism.This reduplicative m alignant circles must be interupted or blocked only by tonsillectom y.

关 键 词：慢性 扁桃体炎 隐窝上皮角蛋白 免疫组织化学 

分 类 号：R766.18[医药卫生—耳鼻咽喉科]