呼吸道合胞病毒融合抑制活性单克隆抗体的鉴定及应用  

Identification and Primary Application of Fusion Inhibiting Active Monoclonal Antibody of Respiratory Syncytial Virus

作　　者：黄升海[1] 史百芬[1] 王明丽[1] 张咏南[1] 胡勇[1] 毕克菊[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032

出　　处：《中国病毒学》2000年第1期34-38,共5页Virologica Sinica

基　　金：安徽省教委资助项目!(95JL0 0 79)

摘　　要：用杂交瘤技术制备了抗呼吸道合胞病毒 (RSV Long株 )的单克隆抗体F3细胞株。经IFA及ELISA证明 ,F3株McAb对RSV抗原特异 ,具中和活性及融合抑制活性 ,其腹水中和效价和融合抑制效价分别为 1∶12 8和 1∶6 4。将F3株McAb与商售混合单抗试剂盒进行临床诊断比较 ,二者阳性符合率 96 .9% ,阴性符合率 10 0 %。Monoclonal antibody of F 3 hybridoma cell strain against RSV specific antigen was prepared by hybridoma and tested by indirect immunofluorescence and ELISA. It has neutralizing and fusion inhibiting activity. The neutralizing titres and fusion inhibiting titres of ascites of mice are 1∶128 and 1∶64 respectively. Clinical diagnosis by comparing McAb of F 3 strain with fixed McAb of commerical kit show that the positive according rate are 96.9% and negative according rate are 100%. F 3 hybridoma cell strain probably produces a monoclonal antibody against fusion protein of RSV.

关 键 词：呼吸道合胞病毒 单克隆抗体 鉴定 融合抑制试验 

分 类 号：R373.1[医药卫生—病原生物学]