牛泡沫病毒两类启动子活性的比较和机制探讨  

Activity Comparison and Mechanism of Two Promoters of Bovine Foamy Virus

作　　者：王世珍[1] 张莉[1] 刘佳建[1] 刘淑红[1] 陈启民[1] 耿运琪[1] 

机构地区：[1]南开大学生命科学院,天津300071

出　　处：《中国病毒学》2000年第1期93-96,共4页Virologica Sinica

基　　金：国家自然科学基金!(39770 0 32 );教育部博士点基金!(980 0 5 5 16 )

摘　　要：Two promoters, LTR (long terminal repeat) and IP (internal promoter), exist in genomes of foamy viruses. Cell transfection and transient expression assay demonstrated that: the basal activity of BFV (bovine foamy virus) IP is much higher than that of BFV LTR; transactivator of BFV—Borf 1, which activates gene expression directed by BFV LTR, also functions on BFV IP with an activation fold higher than that on LTR. The results suggest that BFV IP and LTR may regulate viral gene expression by different mechanisms, and that Borf 1 may stimulate BFV IP and LTR in different ways. In addition, an in vivo DNA competition assay demonstrated that a common transcription factor may be involved in both mechanisms of the two promoters.Two promoters, LTR (long terminal repeat) and IP (internal promoter), exist in genomes of foamy viruses. Cell transfection and transient expression assay demonstrated that: the basal activity of BFV (bovine foamy virus) IP is much higher than that of BFV LTR; transactivator of BFV—Borf 1, which activates gene expression directed by BFV LTR, also functions on BFV IP with an activation fold higher than that on LTR. The results suggest that BFV IP and LTR may regulate viral gene expression by different mechanisms, and that Borf 1 may stimulate BFV IP and LTR in different ways. In addition, an in vivo DNA competition assay demonstrated that a common transcription factor may be involved in both mechanisms of the two promoters.

关 键 词：牛泡沫病毒 长末端重复序列 内部启动子 启动子 

分 类 号：S852.65[农业科学—基础兽医学]