PGN对Aβ1-42寡聚体促进BV2细胞分泌IL-1β的影响  

The influences of peptidoglycan on the secretion of interleukin-1β in BV2 cells activated by amyloid protein β1-42 oligomers

作　　者：姜新[1] 相荣才[2] 张贺敏[1] 陈晓虹[1] 马恩龙[3] 白丽娟[1] 

机构地区：[1]辽宁省人民医院神经内科,110016 [2]中国医科大学基础部生物物理与物理教研室 [3]沈阳药科大学药学院药理教研室

出　　处：《中国神经免疫学和神经病学杂志》2012年第3期211-214,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨前炎介质肽聚糖(peptidoglycan,PGN)激活BV2细胞内吞β淀粉样蛋白(amyloid pro-teinβ,Aβ)1-42寡聚体后,对白细胞介素-1β(IL-1β)分泌的影响及其机制。方法采用细胞株传代法培养BV2细胞替代小胶质细胞,按Klein方法制备Aβ1-42寡聚体。将BV2细胞分为PGN(20μg/mL)组、Aβ1-42寡聚体(0.5μmol/L)组、PGN(20μg/mL)+Aβ1-42寡聚体(0.5μmol/L)组,比较BV2细胞分泌IL-1β水平;将BV2细胞予PGN(20μg/mL)及PGN+SB202190,比较两组IL-1β分泌水平;将不同浓度的PGN(0、5、10、20、40μg/mL)加入BV2细胞中培养,分别检测培养液中IL-1β的水平。采用ELISA方法测定BV2细胞培养液中IL-1β的水平。结果 PGN、Aβ1-42寡聚体、PGN+Aβ1-42寡聚体均可激活BV2细胞分泌IL-1β,分泌IL-1β高峰分别为孵育后24h、12h、24h;孵育后6、12、24h同时间相比,PGN+Aβ1-42寡聚体组BV2细胞分泌IL-1β水平均较PGN组和Aβ1-42寡聚体组明显增多(均P<0.05);PGN激活BV2细胞分泌IL-1β的水平与PGN浓度有剂量依赖关系(P<0.05);丝裂原活化蛋白激酶(MAPK)抑制剂SB202190使BV2细胞分泌IL-1β量明显减少(P<0.01)。结论 PGN可激活BV2细胞分泌IL-1β,且促进Aβ刺激BV2细胞分泌IL-1β增多,p38MAPK抑制剂可抑制IL-1β分泌,推测p38MAPK可能参与BV2细胞分泌IL-1β的过程。Objective To investigate the effect of pro-inflammatory medium, peptidoglycan （PGN）, on the secretion of interleukin-1β （IL-1β） in amyloid protein β 1-42 （Aβ1-42） oligomer activated BV2 cells. Methods BV2 cells were cultivated and replaced microglia by cell passage method. BV2 cells were firstly separated into four groups and PGN （20 μg/mL）, Aβ1-42 oligomer （0.5 μmol/L）, PGN （20 μg/mL） ＋Aβ1-42 oligomer （0.5 μmol/L）, and PGN （20 μg/mL） ＋SB202190, were given respectively. Then, the PGN treated BV2 cells were further divided into 5 groups and treated with different doses of PGN （0, 5, 10, 20, 40μg/mL, respectively）. Aβ1-42 oligomers were prepared according to Klein WL （2002）. The concentration of IL-1β in BV2 cell culture fluid was determined by ELISA. Results IL-1β secretion in BV2 cells could be activated by PGN, Aβ1-42 oligomers and PGN＋Aβ1-42 oligomers, and the IL-1β peak was at the 24 h, 12 h and 24 h, separately. IL-1β concentration was higher in PGN＋Aβ1-42 oligomers group in comparison with PGN and Aβ1- 42 oligomers groups （P〈0.05, P〈0.05, separately）. Compared to control group, the secretion of IL-1β activated by PGN increased significantly （P 〈 0.01 ）. Dose-dependent relationship existed between the concentration of PGN and the IL-1β secretion level in BV2 cells （P〈0. 05）. SB202190, mitogen-activated protein kinase （MAPK） inhibitor, decreased the secretion of IL-1β in BV2 cells （P〈0.01）. Conclusions PGN can activate the secretion of IL-1β in BV2 ceils, and also promote IL-1β secretion in Aβ stimulated BV2 cells. P38 MAPK inhibitor could decrease IL-1β secretion, suggesting p38MAPK may participate in the process of IL-1β secretion in BV2 cells.

关 键 词：肽聚糖 小胶质细胞 淀粉样β1-42蛋白寡聚体 白细胞介素1Β 

分 类 号：R392.1[医药卫生—免疫学]