自噬对葡萄糖转运蛋白4动力学影响分析  

Research on the Dynamics Effects of Autophagy on GLUT4

作　　者：王李洁[1] 吴坚[1] 陈思聪[1] 杨怡[1,2] 孙永红[1] 郑筱祥[1,2] 

机构地区：[1]浙江大学生物医学工程教育部重点实验室,中国浙江杭州310027 [2]浙江大学求是高等研究院,中国浙江杭州310027

出　　处：《生命科学研究》2012年第2期108-112,124,共6页Life Science Research

基　　金：中国博士后科学基金面上资助项目(20100481427);中国博士后科学基金特别资助(201104735)

摘　　要：葡萄糖转运蛋白4(GLUT4)与胰岛素抵抗有着紧密联系,抑制自噬能减缓胰岛素抵抗.为了探讨自噬对胰岛素抵抗方面的作用,现以GLUT4囊泡为动力学模型,通过全内反射荧光显微镜实时观测3T3-L1成熟脂肪细胞中GLUT4囊泡的运动,并采用高斯拟合及相应的搜索算法,从TIRFM时间序列中提取运动轨迹、速度等信息进行统计分析.结果显示:自噬对GLUT4的运动具有一定的影响.抑制自噬后,GLUT4囊泡运动的胰岛素响应程度增强,长距离运动囊泡增多,平均运动速度加快.Insulin resistance can be alleviated when autophagy is inhibited. Glucose transporter 4 has close relationships with insulin resistance. However, the dynamics effects of autophagy on glucose transporter 4 have not been concerned. Using glucose transporter vesicles as kinetic model and combining of Gauss distri- bution regression fitting method with total internal reflection fluorescence microscopy （TIRFM）, the real-time and dynamic movements of GLUT4 vesicles in the 3T3-L1 adipocyte cells were studied. Similarly utilizing this fitting and coordinate with the corresponding search algorithm to the time-lapse images, their moving trajectories were depicted and the results were statistically analyzed. When autophagy was inhibited, the in- sulin sensitivity of GLUT4 vesicles were enhanced, the number of vesicles in long-range movements was in- creased, and also the velocity of vesicles was increased. GLUT4 vesicles. Autophagy has some effects with the movements of

关 键 词：脂肪细胞 葡萄糖转运蛋白4 自噬 动力学分析 

分 类 号：Q334[生物学—遗传学] Q336