CD3/CD28共刺激调节T细胞多重信号转导通路的基因mRNA表达  

Regulation gene mRNA expression of multi-signal transduction pathways by CD3/CD28 cross-linking stimulation in T cells

作　　者：周俐斐[1] 陈峰阳[2] 李晓誉[2] 徐世芳[2] 叶益萍[2] 

机构地区：[1]浙江省肿瘤医院,杭州310022 [2]浙江省医学科学院药物研究所,杭州310013

出　　处：《中国免疫学杂志》2012年第5期393-397,414,共6页Chinese Journal of Immunology

基　　金：浙江省自然科学基金(No.Y2090471);浙江省科技厅计划(2009F20033)资助项目

摘　　要：目的:观察激活T细胞中信号转导通路的基因变化情况。方法:小鼠T细胞体外经CD3/CD28共刺激4小时,提取RNA,以"信号转导通路发现者"PCR芯片分析18个信号转导通路相关的84个关键基因的mRNA改变情况。结果:CD3/CD28共刺激调节了43个基因,其中上调24个,下调19个,涵盖检测的所有18条信号转导通路。结论:多重信号通路共同参与了T细胞的激活过程。Objective：To investigate the expressions of genes associated with multiple signaling pathways in activiated T cell.Methods：RNA from T cells stimulated by CD3/CD28 cross-linking for 4h were examined using mouse Signal Transduction PathwayFinder PCR array which contain 84 genes in 18 signal transduction pathways.Results：43 genes in total 18 signal pathways were regulated in T cells after CD3/CD28 cross-linking stimulation.Among them,24 genes were up-regulated and 19 genes were down-regulated.Conclusion： Multi-signal transduction pathways simultaneously participate in the process of T cells activation.

关 键 词：T细胞 信号转导通路 PCR芯片 

分 类 号：R392.1[医药卫生—免疫学]