猪链球菌2型溶血素基因缺失株构建及其生物学特性分析  

Construction and Characterization of Streptococcus suis type 2 Mutant Strain with Suilysin Gene Deletion

作　　者：吴炜[1] 张晓燕[1] 唐宇龙[1] 方丽华[1] 方维焕[1] 

机构地区：[1]浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州310058

出　　处：《动物医学进展》2012年第5期22-26,共5页Progress In Veterinary Medicine

摘　　要：猪链球菌溶血素(SLY)是较早确定的猪链球菌毒力因子之一,具有细胞毒性,在猪链球菌致病过程中发挥重要作用。利用同源重组技术成功构建了猪链球菌2型(SS2)sly基因缺失的突变株SS2-Δsly,缺失株的体外溶血活性消失,对小鼠脑血管内皮细胞的细胞毒性显著低于亲本菌株(P<0.01),但不影响猪链球菌2型在巨噬细胞中的存活。该菌株可以用于研究SS2溶血素在感染细胞中的作用机制。Suilysin（SLY）,one of the most important virulence factors of Streptococcus suis type 2（SS2）,plays an important role in the pathogenesis by its cytotoxic activity.We constructed a mutant SS2-Δsly with deletion of the sly gene from a clinical SS2 strain 1 105 by homologous recombination.Deletion of the sly gene abolished the hemolytic activity of SS2 and reduced cytotoxicity to Bend3 cell brain epithelial cells by more than 60% as compared with its parent strain.There was no significant difference between the mutant and parent strains in survival within macrophage cell lines Raw 246.7.The SS2-Δsly mutant strain could be used to explore the mechanisms of SLY in infected cells.

关 键 词：猪链球菌2型 溶血素基因缺失株 溶血性 细胞毒性 

分 类 号：S852.611[农业科学—基础兽医学]