牛轮状病毒RT-LAMP检测方法的建立  

Establishment of RT-LAMP for Detection of Bovine Rotavirus

作　　者：李巍[1] 李宁[1] 袁万哲[1] 孙继国[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《动物医学进展》2012年第5期39-43,共5页Progress In Veterinary Medicine

基　　金：河北省科技支撑计划(10220401D)

摘　　要：建立一种适用于牛轮状病毒(BRV)的反转录-环介导等温扩增(RT-LAMP)的快速检测方法。根据GenBank中登录的BRV VP7基因序列,针对其保守区设计了4条引物,利用RT-LAMP方法进行检测,并优化了反应体系与反应时间,检测了该方法的特异性和灵敏度。结果表明,该方法在等温条件下只需要50min就能检测出结果,与普通RT-PCR相比,具有较短的检测时间、良好的特异性和较高的灵敏度。论文针对BRV建立的RT-LAMP快速检测方法操作简便、无需昂贵设备、结果可视,适用于BRV在基层的快速检测。In this study,we established a rapid detection mathod for bovine rotavirus（BRV） by reverse transcription-loop-mediated isothermal amplification assay（RT-LAMP）.According to the published BRV VP7 sequences in GenBank,four primers were designed.Then,reaction system and reaction time were optimized,specificity and sensitivity were detected.It is shown that the virus could be detected within 50 minutes by the method,with ideal specificity,shorter reaction time and higher sensitivity than that by general RT-PCR.The method was easy to operate,which was suitable for rapid detection of BRV.

关 键 词：牛轮状病毒 反转录-环介导等温扩增 反转录-聚合酶链反应 

分 类 号：S852.653[农业科学—基础兽医学]