OsBTF3基因在水稻光合作用和生长发育中的功能  

Functions of OsBTF3 Gene in Regulation of Photosynthesis,Growth and Development in Rice

作　　者：李广旭[1,2] 陈华民[1] 吴茂森[1] 何晨阳[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]辽宁省农业科学院果树研究所,辽宁熊岳115009

出　　处：《作物学报》2012年第5期904-908,共5页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育重大专项(2009ZX08009-044B,2008ZX08001-002)资助

摘　　要：为了揭示转录因子OsBTF3在水稻生长发育中的功能,比较分析了OsBTF3过量表达和RNAi转基因水稻T1代株系叶片生长和株型发育的表型。结果表明,与野生型对照株相比,过量表达株系在叶绿素含量、叶绿体数量、光合速率、叶片大小、株高、节间长方面显著增加或增强;而RNAi株系的明显降低或减弱。OsBTF3基因的增量或减量表达显著影响水稻光合作用、叶片生长和株型发育。说明OsBTF3在水稻生长发育中具有重要调控功能,可能在水稻转基因分子育种方面具有潜在的应用价值。To reveal the regulatory roles of transcription factor OsBTF3 in growth and development of rice,we comparably analyzed the growth and development of the transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene.Results showed that significant increases in chlorophyll content,chloroplast number,photosynthetic rate,leaf size,plant heights and stem internode length were found in T1 generation of transgenic rice lines with the over-expressed gene compared to wild-type control plants,while remarkable decreases in above phenotypes were observed in plants with the RNAi-silenced gene.Over-expression or silencing of transgene OsBTF3 in rice resulted in significant changes in photosynthesis,growth and development of rice.Therefore,OsBTF3 functions as a key regulator in growth and development,and might be potentially used in molecular breeding of rice for improving agronomic traits.

关 键 词：水稻 OsBTF3 转基因 光合作用 生长发育 

分 类 号：S511[农业科学—作物学]