斑茅割手密杂种后代真实性鉴定及遗传分析  

Identification and Genetic Analysis of Hybrid from Cross between Erianthus arundinacius(Retz.)Jesws.and Saccharum spontaneum L

作　　者：刘昔辉[1] 方锋学[1] 高轶静[1] 张荣华[1] 宋焕忠[1] 杨荣仲[1] 方位宽[1] 段维兴[1] 罗霆[1] 张革民[1] 李杨瑞[1] 

机构地区：[1]广西农业科学院甘蔗研究所,广西甘蔗遗传改良重点实验室,中国农业科学院甘蔗研究中心,国家糖料改良中心广西甘蔗品种改良分中心,甘蔗育种与栽培国家地方联合工程研究中心,广西南宁530007

出　　处：《作物学报》2012年第5期914-920,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31101195,30971837,30860150);国家科技支撑计划项目(2007BAD30B00);国家现代农业产业技术体系建设专项(CARS-20-3);广西自然科学基金项目(0991009Z,2010GXNSFB013027,2011GXNSFF018002);广西农科院基金项目(200906Z,200923,G2010005,G2009008,G2009010,桂农科2011YT01,桂农科2012YZ10)资助

摘　　要：以斑茅GXA87-36(♀)与割手密GXS79-9(♂)杂交获得的经形态鉴别为真杂种的后代材料GXAS07-6-1(斑茅割手密杂合体)及其双亲为材料,利用体细胞染色体计数以及SSR、SRAP分子标记对杂种进行真实性鉴定,并利用SRAP分子标记对斑茅GXA87-36、割手密GXS79-9及其杂种后代遗传位点的传递进行分析。结果表明,GXA87-36体细胞染色体数为2n=60,GXS79-9体细胞染色体数为2n=64,其杂种GXAS07-6-1染色体2n=62,其杂交的染色体遗传方式为n+n;筛选出3对SSR引物和5对SRAP引物可分辨后代的真伪;利用71对SRAP引物扩增后代和双亲,检测到1095个遗传位点,多态性位点数为800;母本有279个位点,其中有79%传递给其后代;父本有439个位点,其中有73%传递给后代;用NTSYS-pc2.10e软件计算Jaccard遗传相似系数,双亲间为0.474,后代与母本、父本间分别为0.696、0.752,表明后代GXAS07-6-1偏向父本遗传。Hybridization between different populations is the most common means to enrich genetic variation.Studies on genetic differentiation and characterizations of hybrid are crucial to protect and evaluate genetic resources.In this paper,the hybrid from Erianthus arundinacius（Retz.）Jesws.× Saccharum spontaneum L.were analyzed using SSR and SRAP markers.The results showed that the hybrid GXAS07-6-1 was the true hybrid identified by using three pairs of SSR primers and five pairs of SRAP primers.A total of 1 095 DNA bands were amplified by 71 pairs of selective SRAP primers.Among 1 095 genetic loci,800 were polymorphic.Therefore,the average polymorphic ratio was 73.0% and each pair of SRAP primers yielded 11 polymorphic loci on average.The genetic relationship between the hybrid and its parents analyzed with software NTSYS-pc2.10e showed that the genetic similarity coefficient was 0.474 between the parents,while 0.696 between the hybrid and the female parent and 0.752 between the hybrid and the male parent,suggesting that the hybrid GXAS07-6-1 was more inherited from male parent.

关 键 词：斑茅 割手密 杂种鉴定 SSR SRAP 

分 类 号：S566.1[农业科学—作物学]