棉花组织培养直接胚胎发生和植株再生  

Direct Somatic Embryogenesis and Plant Regeneration from Cotton Tissue Culture

作　　者：王清连 刘方[2] 周云[2] 汤菊香 刘志红[2] 冯喜兰 张宝红[2] 鲁玉贞 

机构地区：[1]河南职技师院,新乡453003 [2]中国农科院棉花研究所,安阳455112

出　　处：《河南职业技术师范学院学报》2000年第1期22-25,共4页Journal of Henan Vocation-Technical Teachers College

基　　金：河南省自然科学基金!资助项目 ( 991 1 60 1 0 7)

摘　　要：选用我国主栽棉花 ( Gossypiumhirsutum L.)品种“中棉所 1 2”,首次直接诱导获得棉花体细胞胚胎发生 ,并获得了再生植株。结果表明 ,激素是影响棉花体细胞胚胎直接发生的重要因素 ,单独使用 ZT可直接诱导获得棉花胚性愈伤组织和胚状体 ,2 ,4 -D的添加虽有利于愈伤组织的形成 ,但却没能直接诱导获得胚性愈伤组织 :IAA的添加削弱了 ZT的诱导效果。下胚轴、子叶和胚根直接诱导获得胚胎发生的能力不同 ,其中以子叶的分化能力最强 ,胚根次之 ,下胚轴较差。在本实验中 ,棉花体细胞胚胎直接发生的最高频率为 1 1 .4 2 %,占诱导获得总愈伤组织的 4 4.4 4%。Embryogenic callus and somatic embryos have been first obtained directly from cotton( Gossypium hirsutum L.)elite variety cv.CRI 12,from which the plants of CRI 12 were regenerated for the first time.The results indicated that hormone was the important factor that effected the direct induction of cotton embryogenesis and plant regeneration.Embryogenic callus and somatic embryos could have been directly obtained on the medium only with ZT;although 2,4 D was advantageous to the induction of cotton callus,embryogenic callus could not be directly obtained on the medium with 2,4 D.Addition of IAA weakened the effects of ZT for the direct induction of cotton embryogenesis.Hypocotyl,cotyledon and radical could be directly induced to produce embryogenic callus and somatic embryos,but the best explant for the direct induction of cotton embryogenesis was cotyledon,followed by hypocotyl.The highest rate of direct cotton embryogenesis was 11.42%.

关 键 词：棉花 胚胎发生 直接诱导 植株再生 组织培养 

分 类 号：S562.035.3[农业科学—作物学]