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作 者:许春华[1] 陈全家[1] 郭忠军[1] 高文伟[1] 苏秀娟[1] 曲延英[1]
出 处:《新疆农业大学学报》2012年第2期107-111,共5页Journal of Xinjiang Agricultural University
基 金:国家转基因专项(2008ZX08005-004);国家自然科学基金项目(31060151)
摘 要:以两种十字花科类短命植物大蒜芥(Sisybrium altissimum L.)和线果芥(Conringia planisiliqua L.)为材料,利用不同浓度的PEG-6000对二者种子和幼苗进行胁迫,统计种子的发芽率并测定苗期叶片相对含水量,从而筛选出合适的PEG-6000胁迫浓度。测定幼苗胁迫后的7种生理生化指标,然后与拟南芥进行比较,综合评价其抗旱性。结果发现,大蒜芥和线果芥幼苗具有较强的抗旱性;脯氨酸、MDA、SOD、POD等指标可作为大蒜芥抗旱性研究的良好参数;脯氨酸、可溶性糖、MDA、SOD、CAT等指标可用来对线果芥进行抗旱性研究。In this study,the Sisybrium altissimum L. and Conringia planisiliqua L. of the two Cruciferous ephemeral plants were used as test materials, whose seeds and seedlings were stressed by PEG-6000 with different concentrations and germination percentage of seeds were counted and the relative moisture content of leaf in seedling period were tested,thus screening the concentration suitable for PEG-6000 stress and determining seven physiological and biochemical indexes after seedlings were stressed then compared them with Arabidopsis thaliana thus comprehensively assessing its drought-resistance. Finally it was dis- covered that the seedlings of Sisybriurn altissirnum L. and Conringia planisiliqua L. had stronger drought resistance ; The indexes of their proline, MDA, SOD, POD could be used as good parameters for drought resistance research of Sisybriurn altissimurn L. ; The indexes of proline, soluble sugar, MDA, SOD,CAT could be used as drought-resistance research of Conringia planisiliqua L.
分 类 号:Q949.748.3[生物学—植物学]
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