卡介苗对大鼠肺巨噬细胞膜的影响  

Effects of BCG on membranes of rat alveolar macrophages

作　　者：胡连平[1] 龚诒芬[1] 费丽华 孙存普[1] 丛建波[1] 吴德昌 

机构地区：[1]北京放射医学研究所,北京100850

出　　处：《中国药理学与毒理学杂志》1990年第3期194-196,共3页Chinese Journal of Pharmacology and Toxicology

摘　　要：本文观察了卡介苗(GCG)对大鼠肺巨噬细胞(AM)膜的影响。实验结果表明:(1)BCG使AM膜的流动性增高。(2)AM经BCG处理后,胞浆的标志酶LDH和溶酶体标志酶β-Glu漏出量增多,说明BCG明显地损伤了AM的胞浆膜和溶酶体膜。(3)AM经BCG刺激后,释放出大量OH·。BCG were injected into Wistar male rats by 18.5 mg/kg body wt through the tail veins and would be injected again by the same dose in 7 d.Then the rats were killed 2 d later.The lungs were lavaged with EDTA-PBS to acquire alveolar macrophages(AM).Membrane-damaging effects of BCG on the AM were observed.After AM of the rats with or without the treatment of BCG were cultured in vitro for 24 h,the amount of lactate dehydrogenase(LDH)and beta-glucuronidase(β-Glu)released from the AM was measured to reflect the degree of the damage of the membranes.The relative fractions of LDH activity in the AM culture supernatant of the rats with and without the treatment of BCG were 35±SD 4.2 and 18.6 ±5.6% respectively,while those of β-Glu 7.0±3.3 and 2.0±1.0% respectively.In order to study thedamaging feature to the membranes further,we observed the AM membrane fluidity of the rats with or without the treatment of BCG by way of fluorescence labelling as well.The results indicated that the AM membrane fluidity of the rats with the treatment of BCG were greater than those without the treatment of BCG.The coefficients of viscosities were 1.55±SD 0.11 and 2.44±0.64 respectively.Soon after AM were stimulated with BCG in vitro,the oxygen free radicals were measured by the technique of electron spin resonance.The results showed that the AM stimulated with BCG released a quite large amounts of hydroxyl radical.

关 键 词：卡介苗 巨噬细胞 细胞膜 药理 

分 类 号：R967[医药卫生—药理学]