幽门螺杆菌粪便抗原检测的临床应用  

Clinical Application of Helicobacter Pylori Feces Antigen Detection

作　　者：周宁 黎冬梅 叶家卫[2] 

机构地区：[1]广州市海珠区妇幼保健院,广东广州510240 [2]广州市儿童医院

出　　处：《中国医学创新》2012年第14期84-85,共2页Medical Innovation of China

摘　　要：目的:评价幽门螺杆菌粪便抗原(HpSA)检测诊断幽门螺杆菌感染的特异性和敏感性。方法:采用酶联免疫分析法检测150例反复腹痛或伴有恶心、呕吐等上消化道症状患儿的幽门螺杆菌粪便抗原,同时以13C尿素呼气试验(13C-UBT)检查作对照,对HpSA酶联免疫法与13C尿素呼气试验(13C-UBT)检查的阳性结果进行比较。结果:150例患儿中,HpSA酶联免疫法阳性率30%,对照法阳性率为29.33%,两法阳性检测率比较差异无统计学意义,HpSA酶联免疫法与对照法比较其敏感度和特异度分别为84.09%和92.45%。结论:HpSA酶联免疫法是一种操作简便、安全、可靠的诊断幽门螺杆菌感染的方法,尤其适用于儿童幽门螺杆菌感染的普查。Objective：To evaluation specificity and sensitivity of Helicobacter pylori stool antigen （HpSA） detection in diagnosis of Helicobacter pylori infection.Methods：Enzyme-linked immunosorbent assay of 150 cases of recurrent abdominal pain or nausea, vomiting and other gastrointestinal symptoms in children on the Helicobacter pylori stool antigen, while ^13C urea breath test （^13C-UBT） for the control check, the HpSA enzyme- linked immunoassay and ^13C urea breath test （^13C-UBT）-positive results were compared to evaluate the HpSA diagnostic sensitivity and specificity of Helicobacter pylori infection. Results：150 cases in children, HpSA ELISA-positive rate of 30%, the positive rate was 29.33% control method, the positive detection rate of these two methods was no significant statistical difference, HpSA ELISA method were compared with the sensitivity and specificity degrees were 84.09% and 92.45%.Conclusion：HPSA ELISA is a simple, safe and reliable method of diagnosis of Helicobacter pylori infection, especially for Helicobacter pylori infection in the census.

关 键 词：幽门螺杆菌 粪便抗原 酶联免疫法 

分 类 号：R446.6[医药卫生—诊断学]