白细胞介素-4基因启动子多态性与广西壮族支气管哮喘患儿易感性和血清总免疫球蛋白E水平的相关性  

Association of Polymorphisms of Interleukin-4 Gene Promoter with Asthma and Serum Total Immunoglobulin E Level in Children from Guangxi Zhuang Autonomous Region

作　　者：林娜[1] 郭瑜修[2] 刘运广[1] 韦叶生[3] 

机构地区：[1]右江民族医学院附属医院儿科,广西百色533000 [2]右江民族医学院附属西南医院儿科,广西百色533000 [3]右江民族医学院附属医院临床检验中心,广西百色533000

出　　处：《实用儿科临床杂志》2012年第9期677-680,683,共5页Journal of Applied Clinical Pediatrics

基　　金：广西教育厅科研项目资助[桂教科研(2009)25号]

摘　　要：目的研究IL-4基因启动子区域-589C/T和-33C/T位点多态性在广西壮族儿童中的分布及与壮族儿童支气管哮喘(哮喘)易感性及血清总IgE水平的相关性。方法采用聚合酶链反应-限制性片段长度多态性方法对健康儿童102例和哮喘患儿72例IL-4基因-589位点和-33位点进行分析,ELISA方法检测2组血清总IgE水平。采用SPSS 14.0软件进行统计学分析。结果1.IL-4-589位点在健康对照组中基因型分布频率为CC 5.9%、CT 23.5%、TT 70.6%,哮喘组为CC 2.8%、CT 19.4%、TT 77.8%;健康对照组等位基因频率为C 17.6%、T 82.4%,哮喘组为C 12.5%、T 87.5%。2.IL-4-33位点在健康对照组和哮喘组中基因型分布和等位基因频率与IL-4-589位点频率分布一致,连锁不平衡值△=0.978。3.哮喘组与健康对照组之间基因型频率和等位基因频率比较差异均无统计学意义(Pa>0.05)。4.哮喘组血清总IgE水平较健康对照组明显增高(P<0.01);各组不同基因型间血清总IgE水平比较差异均无统计学意义(Pa>0.05);3种相同基因型不同组间比较哮喘组血清总IgE水平均较健康对照组高(Pa<0.05)。结论在广西地区壮族儿童人群中,IL-4基因启动子-589位点和-33位点存在多态性,两位点存在连锁不平衡,其多态性与壮族儿童哮喘的易感性无关联,与血清总IgE水平无相关性。Objective To determine the distributing frequency of polymorphisms of interleukin - 4 （ IL - 4） gene promoter region - 589C/T and -33C/T location in children from Guangxi Zhuang Autonomous Region and to investigate the correlation between the distributing frequency of the polymorphism of IL -4 gene promoter region -589C/T and -33C/T location, and the asthma susceptibility and the serum total IgE level in children from Guangxi Zhuang Autonomous Region. Methods The polymorphisms of IL - 4 gene C - 589T and C - 33T lo- cus in 72 asthmatic children and 102 normal controls were analyzed by polymerase chain reaction - restriction fragment length polymorphism. The serum total IgE level was determined using enzyme - linked immunosorbent assay in the 2 groups. The SPSS 14.0 software was used for the statistical analysis. Results 1. The distributing frequencies of genotypes were CC 5.9%, CT 23.5%, TT 70.6% in normal control group, and CC 2.8%, CT 19.4% , TT 77.8% in asthmatic group, respectively. 2. The distributing frequencies of alleles were C 17.6% , T 82.4% in normal control group and C 12.5%, T 87.5% in asthmatic group, respectively. The distributing frequencies of genotypes and al- leles of IL - 4 - 33 locus in normal control group and in asthmatic group were consistent with those of IL - 4 - 589 locus. There was a linkage disequilibrium between 2 locus and the A value was 0. 978.3. There was no significant difference in genotypes and alleles between asthmatic group and normal control group （Po 〉 0.05 ）. 4. It was statistically significant to compare with the serum total IgE levels between the 2 groups （ P 〈 0. 01 ）. There was no significant difference of the serum total IgE level between different genotypes （Pa 〉 0.05 ）. It was found that the se- rum total IgE level in asthmatic group was higher than that in normal control group through the comparison of the serum total IgE level between same genotypes （Pa 〈 0.05 ）. Conclusions There was difference in the polymorphism of IL - 4 gene promo

关 键 词：白细胞介素-4 基因多态性 支气管哮喘 壮族儿童 

分 类 号：R725.6[医药卫生—儿科]