氰戊菊酯宫内暴露对雄性仔鼠雄性特征的影响  

Effects of Intrauterine Fenvalerate Exposure on the Male Characteristics of Offspring Rats

作　　者：杨旸[1] 李晓凤[1] 周义军[1] 

机构地区：[1]上海交通大学医学院公共卫生学院,上海200025

出　　处：《环境与职业医学》2012年第5期321-323,共3页Journal of Environmental and Occupational Medicine

基　　金：国家大学生创新性实验计划(编号:091024858);上海交通大学医学院基金(编号:2008XJ006)

摘　　要：[目的]了解氰戊菊酯宫内暴露对雄性仔鼠雄性特征的影响。[方法]选用SD妊娠大鼠20只,随机分为4组,分别给予0、2、10、50mg/kg氰戊菊酯,于妊娠第12~18天进行灌胃染毒,仔鼠出生后第13天每组随机抽取35只雄性仔鼠观察乳头保留情况。仔鼠断乳后进行窝标准化,每组随机保留25只雄性仔鼠,并于出生第35天处死取材,制备睾丸匀浆,应用比色法测定睾丸组织一氧化氮（nitric oxide,NO）及一氧化氮合酶（nitric oxide synthase,NOS）水平。[结果]雄性仔鼠出生第13天,2mg/kg和10mg/kg染毒组与空白对照组比较,乳头保留数差异无统计学意义（P〉0.05）,而50mg/kg染毒组乳头保留数明显高于空白对照组,差异有统计学意义（P〈0.05）。与空白对照组相比,2mg/kg染毒组NOS活性无明显改变（P〉0.05）,10mg/kg和50mg/kg染毒组NOS活性明显升高,差异有统计学意义（P〈0.05）。各染毒组NO含量与空白对照组相比,差异均无统计学意义（P〉0.05）。[结论]氰戊菊酯宫内暴露对雄性仔鼠雄性特征有明显的类雌激素效应。[ Objective ] To investigate the effect of intrauterine fenvalerate exposure on the male characteristics of offspring rats. [ Methods ] Twenty pregnant SD rats were randomly divided into 4 groups, and intragastrically administrated with 0, 2, 10 and 50 mg/kg of fenvalerate respectively from gestation day 12 to 18. The number of nipple retention was observed of 35 male offspring rats randomly selected from each group on postnatal day 13. And 25 male offspring rats were randomly kept after ablactation in each group and killed on postnatal day 35 to prepare testicular homogenate. The level of nitric oxide （NO） and nitric oxide synthase （NOS） in testicles were measured. [ Results ] Compared with the control group, the number of retained nipples was significantly increased in 50mg/kg group （P〈0.05）, but not in 2 and 10mg/kg groups （P〉0.05）; the activity of NOS was significantly increased in 10 and 50 mg/kg groups （P 〈 0.05）, but not in 2 mg/kg group （P 〉 0.05）. There was no significant difference in the level of NO between the exposed groups and the control group （P〉 0.05）. [ Conclusion ] The intrauterine fenvalerate exposure exhibited obvious estrogenic effects on the male characteristics of offspring rats.

关 键 词：氰戊菊酯 大鼠 妊娠 乳头保留 一氧化氮合酶 

分 类 号：R114[医药卫生—卫生毒理学]