羟基红花黄色素A对培养大鼠海马脑片氧-葡萄糖剥夺模型神经发生的影响  

Effect of hydroxysafflor yellow A on neurogenesis in a rat model of oxygen-glucose deprivation in hippocampal slice cultures

作　　者：秦峥[1,2] 王晓锋[3] 叶华[1,4] 郑学宝[2] 

机构地区：[1]南方医科大学中西医结合临床,广州510515 [2]广东医学院中医研究室,东莞523808 [3]南方医科大学第三附属医院神经内科,广州510630 [4]广东医学院天然药物研究与开发重点实验室,湛江524023

出　　处：《国际脑血管病杂志》2012年第4期263-268,共6页International Journal of Cerebrovascular Diseases

基　　金：基金项目：国家自然科学基金（N030772701）;东莞市科技计划项目（No.2008108101032）

摘　　要：目的 探讨氧-葡萄糖剥夺（oxygen-glucose deprivation,OGD）损伤后,羟基红花黄色素A（ hydroxysafflor yellow A,HSYA）对器官型脑片海马齿状回神经发生的影响.方法 利用3d龄Sprague-awley大鼠制备器官型海马脑片,随机分为空白对照组、生理盐水对照组（OGD后给予lml生理盐水）、大剂量HSYA组（OGD后1ml,0.072 mg/ml HSYA干预）和小剂量HSYA组（OGD后1ml,0.036 mg/ml HSYA干预）,每组6张脑片.巢蛋白和5-溴脱氧尿嘧啶核苷免疫荧光法双标增殖神经干细胞（neural stem cell,NSC）.结果 各组脑片OGD前后不同时间点之间NSC数量均存在显著差异（P均＜0.001）,OGD后各组增殖NSC数量显著减少,3d时细胞增殖明显增多,6d时增殖NSC数量较3d时减少但仍高于OGD前.空白对照组、生理盐水对照组、大剂量HSYA组和小剂量HSYA组增殖NSC数量存在显著差异（F=947.077,P＜0.001）.从各时间点看,除OGD前外,其他各时间点增殖NSC数量均存在下列关系：空白对照组＞大剂量HSYA组＞小剂量HSYA组＞生理盐水对照组（P均＜0.001）.结论 HSYA可减轻OGD对NSC的损伤,促进神经再生.Objective To investigate the effect of hydroxysafflor yellow A （HSYA） on neurogenesis of the dentate gyrus organotypic hippocampal slice cultures after oxygen-glucose deprivation （OGD）.Methods The 3-day-old Sprague Dawley rats were used to making organotypic hippocampal slices.They were randomly divided into four groups：a blank control group,a saline control group （giving 1 mL normal saline after OGD）,a high-dose HSYA group （giving 1 ml,0.072 mg/ml HSYA after OGD）,and a low-dose HSYA group （giving 1 ml,0.036 mg/ml HSYA after OGD） （n =6 slices in each group）.Nestin and 5-bromo deoxyuridine nucleoside imnune fluorescence method were used to double-label the proliferation of neural stem cell （NSC）.Results There were significant differences in NSC numbers among the different time points before and after OGD in slices of each group （all P 〈0.001）.After OGD,the proliferation of NSC numbers reduced significantly in each group,cell proliferation increased siginificantly at day 3,and the proliferation of NSC number at day 6 was lower than that at day 3,but they still higher than those before OGD.There were significant differences in the proliferation of NSC numbers among the blank control,the saline control,high-dose HSYA,and low-dose HSYA groups （F =947.077,P 〈0.001）.Observing from each time point,in addition to before OGD,the proliferation of NSC numbers at all other time points had the following relations：the blank control Group 〉 the high-dose HSYA group 〉 the low-dose HSYA group 〉 the saline control group （all P〈0.001）.Conclusions HSYA may alleviate OGD to the NSC injury and contribute to neurogenesis.

关 键 词：羟基红花黄色素A 脑缺血 神经发生 神经保护药 葡萄糖 海马 时间因素 大鼠 

分 类 号：R285.5[医药卫生—中药学]