薄层-紫外法测定蛇床子素和总线性呋喃香豆素  

TLC-spectrophotometric determination of oesthol and total linearfuranocumarin in Shechuangzi

作　　者：程大敦[1] 雷燕 赵俊[1] 

机构地区：[1]南京药物研究所

出　　处：《中国药学杂志》1990年第3期164-166,共3页Chinese Pharmaceutical Journal

摘　　要：蛇床子样品粉碎,在脂肪提取器中用乙醇提取,点于硅胶板上,以甲苯-乙酸乙酯(9:1)为展开剂。相应部分刮下后,用甲醇洗脱,蛇床子素于λ322nm测定,总线性呋喃香豆素于λ249nm测定。在20～100μg范围内获得良好线性。本法结果与HPLC法相比是一致的。The osthol and total linearfuranocumarin inShechuangzi (Cnidium monnieri) were determi-ned by TLC-spectrophotome tric method. Sam-ple was pulverized and extracted with ethanolin Soxhlet extractor. The extracted solutionwas spotted on silica gel plate with toluene-ethylacetate (9:1) as a developing agent. Therelevant parts of the silica gel were scrapedoff, and extracted with methanol (or ethanol)Osthol was determined at 322nm, and the to-tal linearfuranocumarin at 249nm. The linearrelation was obtained between 20～100μg. Theresults were in agreement with those byHPLC method.

关 键 词：蛇床子 蛇床子素 呋喃香豆素 

分 类 号：R284.1[医药卫生—中药学]