黄芪多糖对糖尿病大鼠肺组织中肾素-血管紧张素系统活性的影响  

Effect of astragalus polysaccharide on renin-angiotensin system activity in diabetic rat lung

作　　者：黄宇倩[1] 罗玉[2,3] 吴文莉[2,3] 张洪茂[2,3] 胡茂清[2,3] 张林[2,3] 

机构地区：[1]四川大学华西临床医学院 [2]成都中医药大学第三附属医院 [3]四川省糖尿病防治中心,四川成都610041

出　　处：《四川医学》2012年第5期746-748,共3页Sichuan Medical Journal

摘　　要：目的探讨黄芪多糖对糖尿病大鼠肺组织中肾素-血管紧张素系统活性的影响。方法取4周龄健康SD大鼠,分为正常对照组、糖尿病模型组、培哚普利组、黄芪多糖组。分别给予胃管灌注生理盐水(正常对照组、模型组)、培哚普利2mg/kg(培哚普利组)、黄芪多糖1g/kg(黄芪多糖组),1次/d,每周灌胃5次。8周后处死大鼠,留取肺组织进行组织匀浆,测定血管紧张素Ⅱ(Angiotensin Ⅱ,AngⅡ)浓度,通过苏木精-伊红染色观察肺组织病理变化,免疫组化和图像分析法检测肺组织中AngⅡ的Ⅰ型受体(Angiotensin Ⅱ receptor Ⅰ,ATIR)的表达。结果①糖尿病模型组肺组织肺泡萎陷、破坏,肺泡间隔增宽,培哚普利组、黄芪多糖组大鼠肺泡结构改善。②AngⅡ浓度:模型组、培哚普利组、黄芪多糖组均显著高于对照组(P<0.05),培哚普利组和黄芪多糖组低于模型组(P<0.05)。③ATIR的表达:糖尿病模型组高于正常对照组(P<0.05),培哚普利组、黄芪多糖组低于模型组(P<0.05)。结论①糖尿病大鼠肺组织中肾素血管紧张素系统活性增加,可能参与了肺组织的损害。②黄芪多糖、培哚普利能降低肾素血管紧张素系统活性,改善肺结构。Objective To investigate the effect of astragalus polysaccharide on renin-angiotensin system activity in diabetic rat lung.Methods Heath SD rats were divided into normal group,model group,perindopril group,astragalus polysaccharide group,and were administered saline（normal and model group）,perindopril 2mg/kg,astragalus polysaccharide1mg/kg,once per day,five times per week.Rats were killed after 8 weeks,lung tissue was homogenated,Angiotensin II was detected.Pathological changes in lung tissue was observed through HE staining.Expression of Angiotensin II receptor I（ATIR） was measured by immunohistochemistry.Results ① Alveolars were collapsed,destructed,alveolar septum was widened in diabetic model rats.Alveolar structure was improved in perindopril and astragalus polysaccharide group.②Angiotensin II concentration： Angiotensin II concentrations in diabetic model,perindopril and astragalus polysaccharide group were higher than that in normal group（P〈0.05）.Angiotensin II concentrations in perindopril and astragalus polysaccharide group were less than that in diabetic model group（P〈0.05）.③Expression of ATIR： The expression of ATIR in diabetic model group was higher than in normal rats（P〈0.05）,in perindopril and astragalus polysaccharide group was less than that in diabetic model group（P〈0.05）.Conclusion Renin-angiotensin system activity elevated in diabetic rat lung tissue,which may participate in the lung damage.Astragalus polysaccharide and perindopril reduced it and improved lung structure.

关 键 词：黄芪多糖 糖尿病肺 肾素-血管紧张素系统 

分 类 号：R285.5[医药卫生—中药学]