He-Ne激光对小鼠联会复合体影响的研究  

Study on Effect of He Ne Laser on Mice Synaptonemal Complex

作　　者：李鸿业[1] 牛新华[2] 徐艳岩[2] 刘文波[1] 曲惠青[3] 

机构地区：[1]滨州医学院电镜室,滨州256603 [2]滨州医学院生物学教研室,滨州256603 [3]滨州医学院附属医院检验科,滨州256603

出　　处：《中国激光》2000年第3期274-278,共5页Chinese Journal of Lasers

摘　　要：以两种不同剂量的He Ne激光照射两组雄性小鼠左侧睾丸。电镜观察以微铺展技术制备的精母细胞联会复合体。结果表明 ,两个试验组小鼠被照射的左侧睾丸精母细胞联会复合体畸变率未见明显升高。联会复合体的畸变类型 ,包括断裂、单侧侧生组份 (LE)断裂、不联会、联会紊乱及圆环等 ,照射后与对照组也无明显差异。试验所用He Ne激光剂量比临床治疗常用剂量大得多。表明He Ne激光照射睾丸治疗疾病不致引起联会复合体畸变。Left testes of male mice in two groups were irradiated by the He Ne laser with two different powers. Synaptonemal complex (SC) of spermatocytes with a surface microspreading technique were examined by the electron microscopy. No significant increase in frequency of SC aberrations was observed in the irradiated left testes of the two experiment groups. After irradiating the types of SC aberrations, involving breakage, LE breakage, asynapsis, synapsis irregularity and ring, were not radically different in the experiment groups from the control. The laser energy density for the experiment was much higher than for clinical treatment. The results show irradiating testes by He Ne laser isn′t able to induce SC aberrations.

关 键 词：HE-NE激光 联会复合体 畸变 细胞遗传学 

分 类 号：Q343.245[生物学—遗传学] Q631