难培养微生物培养方法的研究进展  

Advances in Culture Methods of Oligotrophic Microbes

作　　者：曾建民[1] 曾振顺[1] 原红娟[1] 谭志远[1] 

机构地区：[1]华南农业大学农学院,广州510642

出　　处：《生物技术进展》2012年第3期165-170,共6页Current Biotechnology

基　　金：高校博士点专项基金(20094404110006);国家973计划项目(2010CB126502);广东省科技厅项目(2010B060200017;2010B090400450)资助

摘　　要：利用环境样品16SrRNA克隆测序等许多非培养(culture-independent)研究表明,自然界存在许多独特而难培养未培养的微生物,如何开发更多的微生物资源成为当前重要的课题,本文综述了影响分离得到难培养微生物的主要原因,介绍了当前出现的已经证实卓有成效的新型培养基及培养方法,旨在为研究开发更多创新的培养方法提供参考借鉴。The culture-independent technique of 16S rRNA clone and sequencing in samples of environments suggests that there exists a host of unique and culture-independent microbes in nature.It is an important subject to learn how to explore as many as microbes.In view of that,this paper reviews the mainly reasons why is difficult for us to culture these culture-independent microbes and introduces the innovative culture technique and the improved culture medium those are proved stunning effective recently.By doing that we believe more and more innovative culture technique will show up in near future.

关 键 词：难培养微生物 培养方法 新药物 微观世界 

分 类 号：Q93[生物学—微生物学]