神经干细胞-施万细胞-聚乳酸-羟基乙酸共聚物支架移植对脊髓损伤大鼠的影响  

Effect of Complex of Neural Stem Cells, Schwann Cells,and Poly (Lactic-co-glycolic Acid) Scaffolds Transplant on Spinal Cord Injured Rats

作　　者：夏雷[1] 郝淑煜[2] 李德志[2] 陈刚[3] 高川川[1] 历俊华[4] 万虹[4] 

机构地区：[1]北京三博脑科医院,首都医科大学第十一临床学院,北京市100093 [2]首都医科大学附属北京天坛医院,北京市100050 [3]无锡市第四人民医院神经外科,江苏无锡市214062 [4]首都医科大学附属北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2012年第5期417-419,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金国际合作研究资助项目(30540450581)

摘　　要：目的探讨种植神经干细胞(NSCs)与施万细胞(SCs)的聚乳酸-羟基乙酸共聚物(PLGA)支架移植促进脊髓损伤大鼠神经功能恢复的作用及机制。方法体外培养NSCs和SCs,以PLGA为支架移植入大鼠T8半横断脊髓损伤处。实验动物随机分为PLGA组、PLGA+NSCs组和PLGA+NSCs+SCs组。术前和术后进行皮层运动诱发电位(CMEPs)检查及BBB评分;然后在同侧或对侧进行T6再次半横断,并进行CMEPs检测及BBB评分。结果 CMEPs的恢复率及波幅在PLGA+NSCs+SCs组最高。移植后,大鼠BBB评分逐渐改善;在移植后第2周及以后,PLGA+NSCs组和PLGA+NSCs+SCs组的BBB评分显著高于PLGA组(P<0.001)。同侧再次半横断后,CMEPs消失,BBB评分快速恢复;对侧再次半横断后,大鼠双下肢完全瘫痪。结论种植NSCs和SCs的PLGA支架移植有利于脊髓损伤功能重建,再生轴突可能形成了功能性连接;但是同侧再生轴突对脊髓功能的恢复作用有限。Objective To explore the effect of transplanting poly （lactic-co-glycolic acid） （PLGA） scaffolds seeded with neural stem cells （NSCs） and Schwann cells （SCs） on spinal cord injured rats and the mechanism. Methods NSCs and SCs were cultured in vitro and then seeded into the directional PLGA scaffolds. Then PLGA-cell complexes were implanted into the spinal cord hemisected rats, which were divided into PLGA group, PLGA＋NSCs group and PLGA＋ NSC＋SCs group. The rats were tested with cortical motor evoked poten- tials （CMEPs） and Basso-Beattle-Bresnahan （BBB） score. Then, the rats were further ipsilaterally or contralateral hemisected at T~ and test- ed with CMEPs and BBB score again. Results The incidence of recovery and the amplitudes of CMEPs were the highest in PLGA＋NSCs＋ SCs group. The rats exhibited a gradual improvement in hindlimh locomotor function in score. The BBB score was the least in the PLGA group in the 2nd week or later. After retransected ipsilaterally, the CMEPs disappeared again and the BBB score improved quickly. But after retransected contralaterally, the rats were completely paraplegia. Conclusion The directional PLGA scaffolds seeded with NSCs and SCs fa- cilitate the recovery in spinal cord injured rats, which may associate with axonal regeneration and functional connections, but play a limited role.

关 键 词：脊髓损伤 组织工程 神经干细胞 施万细胞 聚乳酸-羟基乙酸共聚物 支架 

分 类 号：R651.2[医药卫生—外科学]