大黄酚脂质体对脑缺血再灌注损伤小鼠海马神经元凋亡的影响  

Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury

作　　者：宋金艳[1] 张力[1] 赵晓倩[1] 宋志斌[1] 

机构地区：[1]河北北方学院药理教研室

出　　处：《神经药理学报》2011年第2期7-13,共7页Acta Neuropharmacologica

基　　金：河北省自然科学基金资助项目(No.C2009001026)

摘　　要：目的:观察脑缺血再灌注损伤后,大黄酚脂质体对小鼠海马神经元凋亡的影响,探讨大黄酚脂质体对脑缺血再灌注损伤小鼠海马的保护作用及其机制。方法:采用暂时性阻断颈总动脉的方法建立小鼠脑缺血再灌注损伤模型,腹腔注射(ip)大黄酚脂质体10.0,1.0,0.1 mg.kg-1,观察小鼠脑缺血再灌注后神经功能学和病理形态学的改变,并采用免疫组化方法检测海马神经元凋亡相关蛋白半胱氨酰天冬氨酸蛋白酶-3(caspase-3),Bcl-2和Bcl-2-Associated X(Bax)的表达。结果:大黄酚脂质体可明显抑制脑缺血再灌注引起的神经元的丢失,提高Bcl-2的表达,降低caspase-3和Bax的表达,提高神经功能评分,减少病理形态改变,减轻海马神经元损伤,其中以10.0 mg.kg-1组大黄酚脂质体的作用最为明显(P<0.05)。结论:脑缺血再灌注损伤后应用大黄酚脂质体对海马神经元有明显的保护作用,其机制可能与上调Bcl-2的表达,下调caspase-3和Bax的表达有关。Objective：To observe the influence of chrysophanol liposomes on apoptosis of hippocampal neurons in mice and to study the protective effects on cerebral ischemia and reperfusion injury and its mechanism.Methods：Cerebral ischemia and reperfusion injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries.Chrysophanol liposomes（10.0,1.0,0.1 mg·kg-1） was injected intraperitoneally.The effects of chrysophanol liposomes were studied on the neurological function,the pathomorphology of brain tissues and the expression of apoptosis-related protein caspase-3,Bcl-2 and Bax in hippocampal neuron using immunohistochemistry.Results：Chrysophanol liposomes could increase the expression of Bcl-2,decrease the expression of Bax and caspase-3,improve the neurological function score and decrease the pathological change in chrysophanol liposomes treated animals.The effect of chrysophanol liposomes was most obviously in group 10.0 mg·kg-1（P〈0.05）.Conclusion：Chrysophanol liposomes have a significant protective effect on hippocampal neurons after cerebral ischemia and reperfusion injury.The mechanism may be related to increasing the expression of Bcl-2,reducing caspase-3 and Bax.

关 键 词：大黄酚脂质体 脑缺血再灌注损伤 海马 凋亡 半胱氨酰天冬氨酸蛋白酶-3 BCL-2 Bcl-2-Associated X 

分 类 号：R971[医药卫生—药品]