长春花萜类吲哚生物碱的分子生物学与代谢工程研究  

Molecular Biology and Metabolic Engineering of the Indole Pathway in Catharanthus roseus(L.) G.Don

作　　者：卢衍[1,2] 常凯[1,2] 郑月[1,2] 许宏宣[1,2] 杨春贤[1,2] 

机构地区：[1]西南大学三峡库区生态环境教育部重点实验室,重庆400715 [2]西南大学生命科学学院天然产物与代谢工程实验室,重庆400715

出　　处：《安徽农业科学》2012年第16期8793-8796,8808,共5页Journal of Anhui Agricultural Sciences

摘　　要：长春花不仅是重要抗癌药物,也是长春碱与长春新碱的唯一来源,并且还包含有其他多种药用成分。近年来,长春花次生代谢得到了广泛的研究,但仍有许多不明之处。该研究对长春花的研究进展进行了综述,详细阐述了长春花萜类吲哚生物碱合成的2大途径(莽草酸途径和萜类途径),介绍了该途径中已克隆的部分基因及相关酶,总结了近年来长春花TIAs研究中的代谢工程策略,以期为长春花萜类吲哚生物碱的后续研究提供参考依据。Catharanthus roseus is the only source for the powerful antitumor drugs of vinblastine and vincristine,it also contains some other pharmaceutical compounds.Althrough C.roseus has been studied extensively,many details of its alkaloid pathway are still unknown.In this paper,the research advances in C.roseus were reviewed,the indole alkaloid biosynthetic pathways of C.roseus,including shikimic acid pathway and terpenoid pathway,were expounded in details,the enzymes and genes involved in the indole alkaloid biosynthesis were introduced.Finally,the recent research progress of periwinkle TIAs combined with metabolic engineering strategy was summarized.The study aims to offer reference for the follow-up studies on the terpenoids indoles alkaloid of C.roseus.

关 键 词：长春花 萜类吲哚生物碱 合成途径 代谢工程 

分 类 号：S682[农业科学—观赏园艺]