天然抗肿瘤药物鬼臼毒素的生物转化研究  

Study on Biotransformation of Natural Anti-tumor Drug Podophyllotoxin

作　　者：师宝忠[1] 张磊[1] 罗都强[1] 

机构地区：[1]河北大学生物技术研究中心,河北保定071002

出　　处：《安徽农业科学》2012年第16期8877-8878,9109,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31071701);河北省科技支撑项目(11220314)

摘　　要：[目的]筛选能够转化鬼臼毒素的菌株,得到其转化产物并鉴定结构,以期为高抗肿瘤活性天然药物的获取和研究奠定基础。[方法]采用微生物分批转化法对鬼臼毒素进行结构修饰,利用薄层色谱法和高效液相色谱法对鬼臼毒素及其转化产物进行检测,筛选出能够转化鬼臼毒素的菌株,将其中一株能够转化鬼臼毒素的菌株进行扩大培养,对其发酵液进行乙酸乙酯萃取、常规硅胶柱层析分离提取转化产物,应用现代波谱技术鉴定其转化产物结构。[结果]通过转化鬼臼毒素的研究,筛选出能转化鬼臼毒素的菌株为BS.Str-1、BS.Str-2、BS.Str-3、BS.Str-7、BS.Str-18、BS.Str-21和BS.Str-29;对BS.Str-21扩大培养,提取分离得到唯一的转化产物,经鉴定为苦鬼臼毒素。[结论]该研究表明通过生物转化的方法可以获得鬼臼毒素衍生物,该结果为高抗肿瘤活性天然药物的获取奠定了基础。[Objective] This study aimed to select stains to transform podophyllotoxin,isolate the transformation product and identify the structure.[Method] Structure of podophyllotoxin was modified with microbial batch transformation method.Podophyllotoxin and its transformation products were detected by using TLC and HPLC methods to select strains with ability to transform podophyllotoxin.Scale-up fermentation of one podophyllotoxin-transforming strain was carried out and the fermented material was extracted with EtoAc,isolated with conventional silica gel column chromatography,structure of the transformation product was identified by using classic spectroscopy technology.[Result] Based on researches of podophyllotoxin transformation,seven strains with ability to transform podophyllotoxin were selected,including BS.Str-1,BS.Str-2,BS.Str-3,BS.Str-7,BS.Str-18,BS.Str-21 and BS.Str-29.By scale up fermentation,the only transformation product of BS.Str-21 strain was extracted and isolated,which was identified as picropodophyllotoxin.[Conclusion] This study demonstrated that podophyllotoxin derivatives can be obtained by biotransformation methods,which laid the foundation for acquisition of natural drugs with high anti-tumor activity.

关 键 词：抗肿瘤 鬼臼毒素 生物转化 

分 类 号：S188[农业科学—农业基础科学]